Osmotic Volume Flow in the Proximal Tubule of Necturus Kidney
Osmotic Volume Flow in the Proximal Tubule of Necturus Kidney
复制标题
Necturus 肾近端小管的渗透体积流量
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
A. K. Solomon
中科院分区：
文献类型：
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作者：
C. Bentzel;Martin Davies;W. N. Scott;M. Zatzman;A. K. Solomon
采用劈裂滴油技术测量颈管近端部渗透流引起的体积变化。当NaCl浓度接近60 mM时，没有引起体积流动，而等离子体电解质可透过管壁，其表观反射系数为0.69。平均表观水力传导率为0.33 x 10-11 cm3/达因秒，与其他上皮相当。许多分子大小变化很大的脂不溶性非电解质的表观反射系数约为0.5。鉴于对分子大小不敏感，由管状体积变化确定的表观反射系数似乎很可能主要取决于最靠近管腔的细胞间屏障的孔隙度，而很少提供有关测试物质随后命运的信息。
Volume changes due to osmotic flow in the distal portion of proximal tubules of Necturi were measured by the split oil drop technique. In agreement with previous findings no volume flow was induced by NaCl concentrations close to 60 mM. The tubule wall was found to be permeable to plasma electrolytes, which have an apparent reflection coefficient of 0.69. The mean apparent hydraulic conductivity was 0.33 x 10-11 cm3/dyne sec, comparable with other epithelia. A number of lipid-insoluble nonelectrolytes of widely varying molecular size had apparent reflection coefficients of about 0.5. In view of the insensitivity to molecular size it seems likely that apparent reflection coefficients determined from tubular volume changes depend primarily on the porosity of the intercellular barrier closest to the lumen and give little information about the subsequent fate of the test substances.