Binding of HSA to Macromolecular pHPMA Based Nanoparticles for Drug Delivery: An Investigation Using Fluorescence Methods.
Binding of HSA to Macromolecular pHPMA Based Nanoparticles for Drug Delivery: An Investigation Using Fluorescence Methods.
复制标题
DOI：
10.1021/acs.langmuir.8b01015
复制
发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Xiaohan Zhang;P. Chytil;T. Etrych;Weiwei Liu;Letícia Rodrigues;G. Winter;S. Filippov;C. Papadakis-C.-Papa
中科院分区：
文献类型：
--
作者：
Xiaohan Zhang;P. Chytil;T. Etrych;Weiwei Liu;Letícia Rodrigues;G. Winter;S. Filippov;C. Papadakis-C.-Papa
带有胆固醇侧基的两亲性聚（N-（2-羟丙基）甲基丙烯酰胺）共聚物（pHPMA）在磷酸盐缓冲液中自组装成纳米粒（NPs），可用作肿瘤靶向药物载体。我们以前发现，人血清白蛋白（HSA）与纳米粒子的相互作用很弱。然而，由于来自复杂系统的信号重叠，这种结合的机制无法解决。在这里，我们使用荧光标记来区分组分并表征结合：一方面，将荧光染料连接到pHPMA上，从而可以使用荧光寿命相关光谱法在HSA存在下研究NP的扩散行为。另一方面，HSA的内源荧光猝灭揭示了结合的起源，这主要是HSA与胆固醇侧基之间的络合。此外，获得了结合常数。
Amphiphilic poly( N-(2-hydroxypropyl)methacrylamide) copolymers ( pHPMA) bearing cholesterol side groups in phosphate buffer saline self-assemble into nanoparticles (NPs) which can be used as tumor-targeted drug carriers. It was previously shown by us that human serum albumin (HSA) interacts weakly with the NPs. However, the mechanism of this binding could not be resolved due to overlapping of signals from the complex system. Here, we use fluorescence labeling to distinguish the components and to characterize the binding: On the one hand, a fluorescent dye was attached to pHPMA, so that the diffusion behavior of the NPs could be studied in the presence of HSA using fluorescence lifetime correlation spectroscopy. On the other hand, quenching of the intrinsic fluorescence of HSA revealed the origin of the binding, which is mainly the complexation between HSA and cholesterol side groups. Furthermore, a binding constant was obtained.