Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster resonance energy transfer (FRET) studies
Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Forster resonance energy transfer (FRET) studies
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DOI：
10.1023/a:1022947411788
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发表时间：
2003-03-01
影响因子：
2.7
通讯作者：
Visser, AJWG
中科院分区：
文献类型：
--
作者：
Hink, MA;Visser, NV;Visser, AJWG
校正的荧光激发和发射光谱已从从维多利亚水母中分离出的绿色荧光蛋白 (EGFP)、蓝色荧光蛋白 (EBFP)、青色荧光蛋白 (ECFP)、EGFP 和黄色荧光蛋白 (EYFP-黄水晶) 以及从珊瑚物种 Discosoma 中分离出的红色荧光蛋白 (DsRed) 的几种增强变体获得。光谱存储在数据库中。该报告描述了如何使用光谱作为模板来推导任何一对荧光蛋白的临界转移距离。
Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP-citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.