DETECTION OF SUBSTANCE-P IN THE CENTRAL NERVOUS-SYSTEM BY A MONOCLONAL ANTIBODY
DETECTION OF SUBSTANCE-P IN THE CENTRAL NERVOUS-SYSTEM BY A MONOCLONAL ANTIBODY
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DOI：
10.1073/pnas.76.7.3532
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
MILSTEIN, C
中科院分区：
文献类型：
--
作者：
CUELLO, AC;GALFRE, G;MILSTEIN, C
在许多脑区都发现了具有递质样特性的肽。免疫细胞化学和放射免疫测定方法的应用对阐明这些神经系统有很大帮助。讨论了由杂交骨髓瘤产生的大鼠单克隆抗体的研制及其在研究其中一种肽——P物质中的应用。将小鼠骨髓瘤细胞与超免疫大鼠脾细胞融合后分离出的杂交克隆，是通过在培养细胞系中获得单克隆P物质抗体的标准化和永久性来源而得到的。这种抗体在放射免疫测定中能识别低至10 - 20飞摩尔的P物质的羧基末端部分。它与所测试的其他已知哺乳动物脑肽不发生交叉反应。通过免疫荧光，该抗体能特异性地结合，且背景极低，可结合到位于中枢神经系统明确界定的核团中的神经末梢和细胞体上。
Peptides with transmitter-like characteristics are being found in many brain areas. The application of immunocytochemical and radioimmunoassay methods has contributed much to the clarification of these neuronal systems. The development of a rat monoclonal antibody produced by a hybrid myeloma and its application to the study of 1 of these peptides, substance P was discussed. The hybrid clone, isolated after fusion of mouse myeloma cells with hyperimmune rat spleen cells, was obtained by a standardized and permanent source of monoclonal substance P antibodies in a culture cell system. This antibody recognizes the COOH-terminal part of substance P in radioimmunoassay down to 10-20 f[femto]mol. It does not crossreact with other known mammalian brain peptides tested. By immunofluorescence the antibody binds specifically and with a remarkably low background to nerve terminals and cell bodies located in clearly defined nuclear organizations of the CNS.