Ocular surface reconstruction, amniotic membrane, and cultivated epithelial cells from the limbus
Ocular surface reconstruction, amniotic membrane, and cultivated epithelial cells from the limbus
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眼表重建、羊膜和培养的角膜缘上皮细胞
DOI：
10.1136/bjo.87.12.1437-a
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发表时间：
2003
影响因子：
4.1
通讯作者：
S. Kinoshita
中科院分区：
文献类型：
--
作者：
N. Koizumi;S. Kinoshita
羊膜对角膜基质细胞向肌成纤维细胞转化有抑制作用
严重的眼表疾病如Stevens-Johnson综合征和眼瘢痕性类天疱疮，以及广泛的使人衰弱的化学损伤，其核心是利姆布斯的角膜上皮细胞缺乏。这是因为角膜上皮干细胞存在于眼睛的该区域中，这通过存在沃格特栅栏来指示，1并且当角膜缘上皮被破坏时，角膜表面被结膜组织包裹，具有浅表瘢痕和血管形成，通常伴随持续性上皮缺损。为了手术重建这些眼睛，可以进行角膜缘移植-来自同一患者的另一只健康眼睛（自体移植物）或来自供体材料（同种异体移植物），2这种方法在许多情况下都是有益的。然而，自体移植不是双侧损伤的选择，即使它们可行也有缺点，因为它们需要从健康的眼睛中取出相当大的角膜缘移植物，这不是没有风险的。同种异体移植并不理想，因为它们总是有排斥反应的风险。
最近，一种涉及移植培养的角膜缘细胞来治疗干细胞缺陷的眼睛的新方法已经提供了有希望的结果。3 - 7最初的工作建立了这样的前提，即来自健康角膜缘组织的细胞可以在实验室中培养并移植到角膜上。
Amniotic membrane has an inhibitory effect against transformation of keratocytes to myofibroblasts
Severe ocular surface diseases such as Stevens-Johnson syndrome and ocular cicatricial pemphigoid, as well as a wide range of debilitating chemical injuries, have at their core a deficiency of corneal epithelial cells at the limbus. This is because the corneal epithelial stem cells reside in this region of the eye, indicated by the presence of palisades of Vogt,1 and when the limbal epithelium is destroyed the corneal surface becomes enveloped by conjunctival tissue with superficial scarring and vascularisation often accompanying a persistent epithelial defect. To surgically reconstruct these eyes a keratolimbal graft—either from the other, healthy eye of the same patient (autograft) or from donor material (allograft)—can be carried out,2 and this approach has been beneficial in a number of situations. Autografts, however, are not an option for bilateral injuries, and even when they are feasible have drawbacks because they require a fairly large limbal graft be taken from the healthy eye which is not without risk. Allografts are not ideal as they invariably carry the risk of rejection.
Recently, a new approach that involves the transplantation of cultivated corneal limbal cells to treat stem cell deficient eyes has provided promising results.3–7 Initial work established the premise that cells from healthy limbal tissue could be cultivated in the laboratory and grafted onto …