Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos
Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos
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DOI：
10.1073/pnas.1000389107
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发表时间：
2010-02-23
影响因子：
11.1
通讯作者：
Kudoh, Tetsuhiro
中科院分区：
文献类型：
--
作者：
Cruz, Carlos;Maegawa, Shingo;Kudoh, Tetsuhiro
在脊椎动物中，EVX同源区域转录因子编码基因在胚胎发育的后部区域表达，过表达实验揭示了在鱼类和青蛙尾巴发育中的作用。我们分析了EVE1调控后神经发育和轴突形成的分子机制。我们发现EVE1通过两种独立的机制参与躯干和尾部神经外胚层的形成：第一，EVE1通过编码合成视黄酸的酶诱导神经外胚层的后部化；第二，EVE1通过抑制BMP的表达参与后外胚层的神经诱导。此外，eve1可以在组织者缺陷的Ichabod(-/-)突变体中恢复干神经管的形成。我们得出结论：EVE1对于原肠胚外胚层的前后轴和背腹轴的组织是至关重要的，并且还具有促进躯干和尾部的活性。
In vertebrates, Evx homeodomain transcription factor-encoding genes are expressed in the posterior region during embryonic development, and overexpression experiments have revealed roles in tail development in fish and frogs. We analyzed the molecular mechanisms of posterior neural development and axis formation regulated by eve1. We show that eve1 is involved in establishing trunk and tail neural ectoderm by two independent mechanisms: First, eve1 posteriorizes neural ectoderm via induction of aldh1a2, which encodes an enzyme that synthesizes retinoic acid; second, eve1 is involved in neural induction in the posterior ectoderm by attenuating BMP expression. Further, eve1 can restore trunk neural tube formation in the organizer-deficient ichabod(-/-) mutant. We conclude that eve1 is crucial for the organization of the anteroposterior and dorso-ventral axis in the gastrula ectoderm and also has trunk-and tail-promoting activity.