Effects of ethidium bromide on the mitochondrial adenosine triphosphatase from Trypanosoma cruzi.
Effects of ethidium bromide on the mitochondrial adenosine triphosphatase from Trypanosoma cruzi.
复制标题
溴化乙锭对克氏锥虫线粒体三磷酸腺苷酶的影响。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
S. Ao
中科院分区：
文献类型：
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作者：
Cataldi de Flombaum Ma;S. Ao
克氏锥虫在溴化乙锭（EB; 1.0-2.5 μ M）存在下培养5天，影响Fo/F1-ATP酶复合物的生物合成，推测是通过EB干扰大环DNA编码的推定Fo-扇区亚基的表达。在体外，EB通过Fo/F1-ATP酶复合物和可溶性F1抑制ATP水解，前一种制剂受到的影响最大（EB I50，250 μ M）。分析EB效应在MgATP浓度增加，以及EB和p-氯汞苯甲酸盐抑制的组合，表明该染料与ATP酶在催化位点以外的其他位点相互作用。体外ATP酶抑制与EB对寄生虫生长的影响之间没有发现相关性。
Culturing of Trypanosoma cruzi in the presence of ethidium bromide (EB; 1.0-2.5 microM), for 5 days, affected the biogenesis of the Fo/F1-ATPase complex, presumably by EB interference with the expression of the putative Fo-sector subunits encoded by maxicircle DNA. In vitro, EB inhibited ATP hydrolysis by the Fo/F1-ATPase complex and also by soluble F1, the former preparation being the most strongly affected (EB I50, 250 microM). Analysis of EB effect at increasing MgATP concentrations, as well as combination of EB and p-chloromercuribenzoate inhibitions, indicated that the dye interacts with ATPase at sites other than the catalytic ones. No correlation was found between the in vitro inhibition of ATPase and EB effect on parasite growth.