PHYLOGENY OF SPECIES IN THE FAMILY NEISSERIACEAE ISOLATED FROM HUMAN DENTAL PLAQUE AND DESCRIPTION OF KINGELLA-ORALE SP-NOV
PHYLOGENY OF SPECIES IN THE FAMILY NEISSERIACEAE ISOLATED FROM HUMAN DENTAL PLAQUE AND DESCRIPTION OF KINGELLA-ORALE SP-NOV
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DOI：
10.1099/00207713-43-3-490
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
ZAMBON, JJ
中科院分区：
文献类型：
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作者：
DEWHIRST, FE;CHEN, CKC;ZAMBON, JJ
从每毫升含有 1 杯克林霉素的所谓腐蚀艾肯菌选择性培养基中回收的 14 株人类牙周分离株显示出与腐蚀艾肯菌不同的生化特征。这些生物体是革兰氏阴性杆菌，会腐蚀琼脂。分离株氧化酶呈阳性，脲酶、吲哚和七叶苷呈阴性。它们在过氧化氢酶、硝酸盐还原、赖氨酸脱羧酶和鸟氨酸脱羧酶活性方面与腐蚀大肠杆菌不同。一种分离株，菌株 UB-294，被推定为脱氮金氏菌。第二种分离株，菌株 UB-204，与腐蚀大肠杆菌不同，过氧化氢酶呈阳性，硝酸盐还原呈阴性。包括菌株 UB-38 T（T = 型菌株）在内的 12 个分离株在表型上与 Kingella kingae 相似，只是它们不从麦芽糖中产生酸并且不具有 β 溶血性。确定了菌株 UB-38T、UB-204 和 UB-294 以及动物奈瑟氏菌、犬奈瑟氏菌、脱氮奈瑟氏菌、长奈瑟氏菌、淡黄奈瑟氏菌、猕猴奈瑟氏菌和多糖奈瑟氏菌的典型菌株的基本完整（1,480 个碱基）16S rRNA 序列。将这些序列与先前发表的属于奈瑟菌科的其他六个物种的序列进行了比较。根据比较序列分析的结果，UB-294被确认为反硝化克雷伯菌菌株，UB-204被鉴定为可能属于Eikenella属的新种的成员，UB-38T被鉴定为Kingella属的新种的成员，我们建议将其命名为Kingella orale。由于菌株UB-204是新物种的唯一代表，因此未对其进行命名。描述了用于基于 16S rRNA 序列信息识别 E. corrodens、K. denitificans 和 K. orale 的 DNA 探针。
Fourteen human periodontal isolates recovered from a purported Eikenella corrodens-selective medium containing 1 mug of clindamycin per ml displayed biochemical traits which differed from those described for E. corrodens. These organisms were gram-negative rods which corroded agar. The isolates were oxidase positive and urease, indole, and esculin negative. They differed from E. corrodens in catalase, nitrate reduction, lysine decarboxylase, and ornithine decarboxylase activities. One isolate, strain UB-294, was presumptively identified as Kingella denitrificans. A second isolate, strain UB-204, differed from E. corrodens by being catalase positive and nitrate reduction negative. Twelve isolates, including strain UB-38 T (T = type strain), were phenotypically similar to Kingella kingae except that they did not produce acid from maltose and were not beta-hemolytic. Essentially complete (1,480-base) 16S rRNA sequences were determined for strains UB-38T, UB-204, and UB-294 and the type strains of Neisseria animalis, Neisseria canis, Neisseria denitrificans, Neisseria elongata, Neisseria flavescens, Neisseria macaca, and Neisseria polysaccharea. These sequences were compared with the previously published sequences of six other species belonging to the family Neisseriaceae. On the basis of the results of the comparative sequence analysis, UB-294 was confirmed as a K. denitrificans strain, UB-204 was identified as a member of a new species which may belong in the genus Eikenella, and UB-38T was identified as a member of a new species of the genus Kingella, for which we propose the name Kingella orale. Since strain UB-204 was the only representative of a new species, it was not named. DNA probes for identification of E. corrodens, K. denitrificans, and K. orale based on 16S rRNA sequence information are described.