The taste bud and its innervation in the rat as studied by immunohistochemistry for PGP 9.5
The taste bud and its innervation in the rat as studied by immunohistochemistry for PGP 9.5
复制标题
DOI：
10.1679/aohc.59.317
复制
发表时间：
1996-10-01
影响因子：
--
通讯作者：
Yoshie, S
中科院分区：
文献类型：
--
作者：
Kanazawa, H;Yoshie, S
用免疫组织化学方法在光镜和电镜水平观察大鼠腭状乳头内的味蕾，并进行常规透射电镜观察。PGP9.5（paragonons的标记蛋白）的免疫反应位于III型或味觉细胞的细胞质中。免疫组化和常规电镜观察显示，神经纤维呈较强的免疫反应性，但仍有部分纤维未被染色，形成一个粗糙的上皮下神经丛，向上发出分支穿过基底膜。在味蕾的基部，这些纤维形成了一个迄今为止未知的密集和微妙的网络的胚芽内丛。这个神经丛反过来又在味蕾细胞之间延伸出珠状纤维，与III型细胞形成突触接触。光镜观察证实部分免疫反应神经纤维可到达味孔，电镜观察不能证实其直接暴露于味孔空间。本研究还涉及大鼠味蕾的精细结构，特别是Ⅲ型细胞及其与神经纤维的突触，因为大鼠近年来很少用于这一领域的研究。
The taste bud in the rat vallate papillae was observed by immunohistochemistry for PGP 9.5 at the light and electron microscope levels; routine transmission electron microscopy was also performed. Immunoreactivity for PGP 9.5, a marker protein of paraneurons, was localized in the cytoplasm of the Type III or gustatory cells. More intensive immunoreactivity occurred in the nerve fibers, though a part of the nerve fibers remained unstained.The nerve fibers as detected by the immunostaining and by routine electron microscopy formed a coarse subepithelial plexus which issued branches upwards through the basal lamina. In the basal portion of the taste bud, these fibers formed a hitherto unknown intragemmal plexus of dense and delicate meshwork. This plexus, in turn, extended beaded fibers between the taste bud cells, forming synaptic contacts with Type III cells. Some of the immunoreactive nerve fibers were confirmed to reach the taste pore by light microscopy; electron-microscopic examination could not demonstrate their directly being exposed to the space of the taste pore.This study also deals with the fine structure of the rat taste bud with special reference to the Type III cells and their synapses with nerve fibers, as the rat has recently been rather seldom used in this field of study.