Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface
Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface
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DOI：
10.1016/j.jasms.2007.05.018
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发表时间：
2007-09-01
影响因子：
3.2
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
--
作者：
Page, Jason S.;Kelly, Ryan T.;Smith, Richard D.
研究了电喷雾电离（ESI）界面的电离和传输效率，以进一步了解这些因素如何影响质谱（MS）灵敏度。此外，还研究了ES发射器距离对进口、溶液流速和进口温度的影响。通过将界面前表面与加热的进口毛细管内壁电隔离，可以区分两个表面的损耗，从而实现了整个ESI界面ES电流损耗的定量测量。此外，通过将直径340 μ m的电极线性阵列放置在入口毛细管入口附近，对ESI界面前表面损失的ES电流进行了空间分布。以气相离子形式传输的电流通过单四极杆质谱仪测量灵敏度，从带电液滴和溶剂团簇中进行了区分。研究揭示大型抽样效率到进气毛细管(> 90%在发射器距离我mm),全球而不是当地的气体动态影响ES羽毛的形状产生的气体流导限制进口的毛细管,大型(> 80%)损失的分析物离子传输通过入口后因不完整的反溶剂的溶液流量1.0μL / min,和减少分析物离子峰强度较低的加热温度下,尽管大大提高了ES电流传输效率。
The ionization and transmission efficiencies of an electrospray ionization (ESI) interface were investigated to advance the understanding of how these factors affect mass spectrometry (MS) sensitivity. In addition, the effects of the ES emitter distance to the inlet, solution flow rate, and inlet temperature were characterized. Quantitative measurements of ES current loss throughout the ESI interface were accomplished by electrically isolating the front surface of the interface from the inner wall of the heated inlet capillary, enabling losses on the two surfaces to be distinguished. In addition, the ES current lost to the front surface of the ESI interface was spatially profiled with a linear array of 340-mu m-diameter electrodes placed adjacent to the inlet capillary entrance. Current transmitted as gas-phase ions was differentiated from charged droplets and solvent clusters by measuring sensitivity with a single quadrupole mass spectrometer. The study revealed a large sampling efficiency into the inlet capillary (> 90% at an emitter distance of I mm), a global rather than a local gas dynamic effect on the shape of the ES plume resulting from the gas flow conductance limit of the inlet capillary, a large (> 80%) loss of analyte ions after transmission through the inlet arising from incomplete desolvation at a solution flow rate of 1.0 mu L/min, and a decrease in analyte ions peak intensity at lower temperatures, despite a large increase in ES current transmission efficiency.