Sensitive and stable Cookson-type reagent for derivatization of conjugated dienes for high-performance liquid chromatography with fluorescence detection
Sensitive and stable Cookson-type reagent for derivatization of conjugated dienes for high-performance liquid chromatography with fluorescence detection
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灵敏、稳定的 Cookson 型试剂，用于共轭二烯的衍生化，用于高效液相色谱荧光检测
DOI：
10.1016/0021-9673(92)85266-v
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发表时间：
1992
影响因子：
4.1
通讯作者：
T. Mizuguchi
中科院分区：
文献类型：
--
作者：
K. Shimada;T. Mizuguchi
以6-甲氧基-2-苯基苯并恶唑为荧光基团，合成了一种灵敏、稳定的Cookson型试剂4-取代1，2，4-三唑啉-3，5-二酮，用于共轭二烯的高效液相色谱测定。该试剂通过升华纯化得到稳定的紫色晶体。该试剂在冰冷却下在5分钟内定量产生与维生素D3原的加合物，其对荧光检测高度响应[每次注射的检测限为2 fmol（信噪比= 5）]。
A sensitive and stable Cookson-type reagent, 4-substituted 1,2,4-triazoline-3,5-dione, having 6-methoxy-2-phenylbenzoxazole as a fluorophore, was prepared for high-performance liquid chromatographic measurements of conjugated dienes. The reagent was purified by sublimation to give stable purple crystals. The reagent quantitatively produced the adduct with provitamin D3in 5 min under ice cooling, which was highly responsive to fluorescence detection [detection limit 2 fmol per injection (signal-to-noise ratio = 5)].