Computerized analysis of tumor cells flowing in a parallel plate chamber to determine their adhesion stabilization lag time.
Computerized analysis of tumor cells flowing in a parallel plate chamber to determine their adhesion stabilization lag time.
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DOI：
10.1002/cm.970260109
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发表时间：
1993
影响因子：
--
通讯作者：
J. Patton;D. Menter;D. Benson;G. Nicolson;L. McIntire
中科院分区：
文献类型：
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作者：
J. Patton;D. Menter;D. Benson;G. Nicolson;L. McIntire
细胞黏附在各种生理现象中的重要性，需要对这些行为背后的因素和分子机制有所了解。细胞黏附是一个多步骤的过程，包括初级受体与配体的相互作用，以及随后可能导致焦点接触形成的次级事件。由于缺乏明确的分析方法来研究黏附稳定，对这一过程知之甚少，除了它可能涉及信号事件、受体募集，以及我们已经证明的由细胞膜相关的谷氨酰胺转氨酶引起的共价肽交联[Menter等人：细胞生物物理学]。1992年18：123-143)。为了研究稳定化过程，我们开发了一种采用平行平板流动室并结合视频显微镜和数字图像处理的动态分析方法。我们的研究利用了小麦胚凝集素选择的人类转移性黑色素瘤细胞变种，这些变种在实验转移潜力和转谷氨酰胺酶表达方面表现出差异。使用这种方法，细胞-底物稳定性的定量被发现是快速、可靠、可重复性的，并且在评估阻断这一过程的试剂方面是有用的。
The importance of cell adhesion in a variety of physiological phenomena requires development of an understanding of the factors and molecular mechanisms underlying these behaviors. Cell adhesion is a multistep process involving primary receptor-ligand interactions followed by secondary events that may lead to the formation of focal contacts. Due to the lack of well-defined assays to study adhesion stabilization, little is known about this process, except that it may involve signaling events, receptor recruitment, and, as we have demonstrated, covalent peptide cross-linking by cell membrane-associated transglutaminase [Menter et al.: Cell Biophys. 18:123-143, 1992). To study the stabilization process we have developed a dynamic assay employing a parallel plate flow chamber coupled with video microscopy and digital image processing. Our studies utilize wheat germ agglutinin-selected human metastatic melanoma cell variants that exhibit differences in their experimental metastatic potential and expression of transglutaminase. Using this assay, quantifying cell-substrate stabilization was found to be quick, reliable, reproducible, and useful in evaluating agents that block this process.