Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
复制标题
使用由可生物降解弹性体聚（3-羟基丁酸酯-co-3-羟基戊酸酯）制成的纳米纤维网预防过度疤痕形成
DOI：
10.1177/2041731420949332
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发表时间：
2020-08-01
影响因子：
8.2
通讯作者：
Han, Jing
中科院分区：
文献类型：
--
作者：
Kim, Hye Sung;Chen, Junyu;Han, Jing
为了减少伤口愈合中的过度瘢痕形成，制造由卤古菌产生的生物可降解弹性体聚（3-羟基丁酸酯-共-3-羟基戊酸酯）（PHBV）组成的静电纺丝纳米纤维网，用作伤口敷料。三种具有不同3 HV含量的PHBV聚合物用于制备溶液浇铸膜或静电纺丝纳米纤维网。随着3 HV含量的增加，结晶度降低，支架变得更有弹性。纳米纤维网表现出比膜更大的弹性和断裂伸长率。当用于体外培养人真皮成纤维细胞时，PHBV网片提供更好的细胞附着和增殖，较少分化为肌成纤维细胞，并且较少基底收缩。在全层小鼠伤口模型中，用薄膜或补片治疗能够使苍白的薄组织再生，而没有结痂、脱水或结核性瘢痕形成。用补片治疗的伤口的表皮在2周内在真皮中形成小的内陷，表明毛囊和汗腺再生。与体外结果一致，补片通过下调α-SMA和TGF-β1以及上调TGF-β3减少体内肌成纤维细胞分化。用网状物处理的再生伤口比用薄膜处理的再生伤口更柔软和更有弹性。这些结果表明，静电纺丝纳米纤维PHBV网通过调节肌成纤维细胞形成来减轻过度的瘢痕形成，显示出其用作伤口敷料的前景。
To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cast films or electrospun nanofibrous meshes. As 3HV content increases, the crystallinity decreases and the scaffolds become more elastic. The nanofibrous meshes exhibit greater elasticity and elongation at break than films. When used to culture human dermal fibroblasts in vitro, PHBV meshes give better cell attachment and proliferation, less differentiation to myofibroblasts, and less substrate contraction. In a full-thickness mouse wound model, treatment with films or meshes enables regeneration of pale thin tissues without scabs, dehydration, or tubercular scar formation. The epidermis of wounds treated with meshes develop small invaginations in the dermis within 2 weeks, indicating hair follicle and sweat gland regeneration. Consistent with the in vitro results, meshes reduce myofibroblast differentiation in vivo through downregulation of α-SMA and TGF-β1, and upregulation of TGF-β3. The regenerated wounds treated with meshes are softer and more elastic than those treated with films. These results demonstrate that electrospun nanofibrous PHBV meshes mitigate excessive scar formation by regulating myofibroblast formation, showing their promise for use as wound dressings.