A novel cytosensor based on Pt@Ag nanoflowers and AuNPs/Acetylene black for ultrasensitive and highly specific detection of Circulating Tumor Cells
A novel cytosensor based on Pt@Ag nanoflowers and AuNPs/Acetylene black for ultrasensitive and highly specific detection of Circulating Tumor Cells
复制标题
一种基于 Pt@Ag 纳米花和 AuNPs/乙炔黑的新型细胞传感器，用于循环肿瘤细胞的超灵敏和高特异性检测
DOI：
10.1016/j.bios.2018.01.001
复制
发表时间：
2018
影响因子：
12.6
通讯作者：
Feng Wenli
中科院分区：
文献类型：
--
作者：
Tang Sitian;Shen Huawei;Hao Yixiong;Huang Zhenglan;Tao Yiyi;Peng Yang;Guo Yongcan;Xie Guoming;Feng Wenli
作为转移的细胞来源的循环肿瘤细胞（CTC）是从原发性肿瘤脱离并在外周血中循环的癌细胞。它们提供了有关肿瘤表型的丰富信息。在这里，这项工作报告了一种新的超灵敏免疫测定方案，用于检测CTC，通过使用Pt@Ag纳米花（Pt@AgNFs）和AuNPs/乙炔黑（AuNPs/AB）纳米材料。在所建立的方法中，AuNPs/AB纳米材料被用作基底材料，以增加比表面积和增强金电极的导电性。蛋白G用于捕获抗体的定向固定，这大大提高了MCF-7细胞的捕获效率。本课题组创新性合成的Pt@AgNFs具有高的比表面积和良好的生物相容性，不仅可以作为信号抗体（Ab 2）的载体，还可以催化H2 O2的还原，有效地放大电流信号。电流信号与CTC浓度在20 ~ 1× 106 cells mL-1范围内呈线性关系，在稳定性和重现性良好的条件下，检出限可低至3cells mL-1。此外，所提出的细胞传感器在检测人血液样品中的CTC中显示出优异的性能。这些结果表明，所提出的细胞传感器将是一个有前途的应用准确定量检测CTC。
Circulating tumor cells (CTCs), as the cellular origin of metastasis, are cancer cells that break away from a primary tumor and circulate in the peripheral blood. And they provide a wealth of information about tumor phenotype. Here, this work reported a novel ultrasensitive immunoassay protocol for the detection of CTCs by using Pt@Ag nanoflowers (Pt@AgNFs) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial. In the established approach, AuNPs/AB nanomaterial was used as substrate material to increase the specific surface area and enhance the conductivity of the gold electrode. Protein G was used for oriented immobilization of capture antibody, which strongly improved the capture efficiency of MCF-7 cells. The innovatively synthesized Pt@AgNFs by our group with high specific surface area and good biocompatibility were not only as the carriers of signal antibodies (Ab2) but also catalyzed the reduction of H2O2, which effectually amplified the current signal. A linear relationship between current signals and the concentrations of CTCs was obtained in the range from 20 to 1×106cells mL−1and the detection limit is as low as 3 cells mL−1on condition of acceptable stability and reproducibility. Furthermore, the as-proposed cytosensor showed excellent performance in the detection of CTCs in human blood samples. These results suggest that the proposed cytosensor will be a promising application for accurately quantitative detection of CTCs.