ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION
ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION
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DOI：
10.1021/ja00127a026
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发表时间：
1995-06-07
影响因子：
15
通讯作者：
KUNITAKE, T
中科院分区：
文献类型：
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作者：
LVOV, Y;ARIGA, K;KUNITAKE, T
多层膜，其中包含有序层的一个以上的蛋白质物种组装通过交替的静电吸附主要与带正电荷的聚（乙烯亚胺）（PEI）或带负电荷的聚（苯乙烯磺酸盐）（PSS）。使用的水溶性蛋白质是细胞色素c（Cyt）、肌红蛋白（Mb）、溶菌酶（Lys）、组蛋白f3、血红蛋白（Hb）、葡糖淀粉酶（GA）和葡萄糖氧化酶（GOD）。带电荷的蛋白质层与线性聚合物形成多层，线性聚合物充当胶或填料。通过石英晶体微量天平和UV光谱监测组装。线性膜生长观察到至少25个分子层。Mb和Lys（均带正电荷）的组装以{PEI/PSS +（Mb/PSS）（2）+（Mb/PSS/Lys/PSS）（4）}的形式与PSS交替实现。带相反电荷（pH 6.5）的Lys和GOD的组装体由通过聚阳离子/聚阴离子双层分离的Lys和GOD层组成：{PEI/PSS/PEI +（PSS/Lys）（2）+ PSS/PEI +（GOD/PEI）（6）}。Hb在pH 4.5时组装为“阳性"单位（与PSS交替），在pH 9.2时组装为”阴性"单位（与PEI交替）。还组装了由交替的蒙脱土、PEI和GOD层组成的多层。这些生物分子结构开辟了一条构建人工协调蛋白质系统的道路，该系统可以进行复杂的酶促反应。
Multilayer films which contain ordered layers of more than one protein species were assembled by means of alternate electrostatic adsorption mostly with positively charged poly(ethylenimine) (PEI) or with negatively charged poly(styrenesulfonate) (PSS). Water-soluble proteins used are cytochrome c (Cyt), myoglobin (Mb), lysozyme (Lys), histone f3, hemoglobin (Hb), glucoamylase (GA), and glucose oxidase (GOD). Charged protein layers formed multilayers with linear polymers acting as glue or filler. The assembly was monitored by a quartz crystal microbalance and UV spectroscopy. Linear film growth was observed up to at least 25 molecular layers. The assembly of Mb and Lys, both positively-charged, was realized in alternation with PSS in the form of {PEI/PSS + (Mb/PSS)(2) + (Mb/PSS/Lys/PSS)(4)}. The assembly of oppositely-charged (at pH 6.5) Lys and GOD consists from Lys and GOD layers separated by a polycation/polyanion bilayer: {PEI/PSS/PEI + (PSS/Lys)(2) + PSS/PEI + (GOD/PEI)(6)}. Hb was assembled as ''positive'' unit at pH 4.5 (in alternation with PSS) and as ''negative'' unit at pH 9.2 (in alternation with PEI). A multilayer consisting of alternating montmorillonite, PEI, and GOD layers was also assembled. These biomolecular architecture open a way to construct artificially orchestrated protein systems that can carry out complex enzymic reactions.