CD10 - A valuable tool for the light microscopic diagnosis of microvillous inclusion disease (familial microvillous atrophy)
CD10 - A valuable tool for the light microscopic diagnosis of microvillous inclusion disease (familial microvillous atrophy)
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DOI：
10.1097/00000478-200207000-00008
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发表时间：
2002-07-01
影响因子：
5.6
通讯作者：
Livne, E
中科院分区：
文献类型：
--
作者：
Groisman, GM;Amar, M;Livne, E
微绒毛包涵体病(MID)是一种特殊的肠道刷状缘疾病，导致婴儿难治性分泌性腹泻。目前。需要进行电子显微镜分析才能明确诊断。然而，这项技术并不总是可用的或可行的，诊断的微绒毛夹杂可能在所有标本中都不明显。因此，一组显示MID特定染色模式的组织化学和免疫组织化学染色的可用性将使病理学家能够在不借助电子显微镜的情况下对这种疾病做出明确的诊断。CD10是一种膜相关的中性多肽酶，在正常小肠中呈线状刷状边缘染色。本文研究了6例MID患者和24例正常对照(10例正常小肠和10例乳糜泻)小肠组织中CD10的染色情况。自身免疫性肠病2例，过敏性肠病2例)。所有MID病例表面肠上皮细胞胞浆CD10免疫反应阳性。与之相反，所有对照病例均呈线状刷缘染色。高碘酸席夫、多克隆癌胚抗原和碱性磷酸酶也得到了类似的结果。已知的三种染色显示MID表面的肠细胞胞浆染色。结论：CD10是诊断MID的有价值的工具。它可以用作包括MID中具有独特染色模式的其他染色的小组的一部分，例如高碘酸希夫、多克隆癌胚抗原和碱性磷酸酶。我们认为，当顽固性腹泻婴儿的小肠活检显示表面肠上皮细胞胞浆染色上述染色时，即可明确诊断为MID。
Microvillous inclusion disease (MID) is a specific disorder of the intestinal brush border that leads to intractable secretory diarrhea in infants. At present. electron microscopic analysis is required for its definitive diagnosis. However, this technique is not always available or feasible, and the diagnostic microvillous inclusions may not be evident in all specimens. Accordingly, the availability of a panel of histochemical and immunohistochemical stains displaying a specific staining pattern for MID will allow pathologists to reach a definitive diagnosis of this disorder without recourse to electron microscopy. CD 10 is a membrane-associated neutral peptidase, shown to have a linear brush-border staining pattern in normal small intestine. We studied the staining pattern of CD 10 in small intestinal biopsies from six patients with MID and in 24 control cases (10 normal small intestine, 10 celiac disease. two autoimmune enteropathy, and two allergic enteropathy). All MID cases revealed prominent cytoplasmic CD10 immunoreactivity in surface enterocytes. In contrast, all control cases showed linear brush-border staining. Similar results were obtained with periodic acid-Schiff, polyclonal carcinoembryonic antigen, and alkaline phosphatase. three stains known to show cytoplasmic staining of surface enterocytes in MID. In conclusion, CD10 is a valuable tool for the diagnosis of MID. It may be used as part of a panel that includes other stains with a distinctive staining pattern in MID such as periodic acid-Schiff, polyclonal carcinoembryonic antigen, and alkaline phosphatase. We suggest that the definitive diagnosis of MID can be reached when small bowel biopsies from infants with intractable diarrhea display cytoplasmic staining of surface enterocytes with the above-mentioned stains.