Neutrophil extracellular trap formation and extracellular DNA in sputum of stable COPD patients
Neutrophil extracellular trap formation and extracellular DNA in sputum of stable COPD patients
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DOI：
10.1016/j.rmed.2015.08.008
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发表时间：
2015-10-01
影响因子：
4.3
通讯作者：
Watz, Henrik
中科院分区：
文献类型：
--
作者：
Pedersen, Frauke;Marwitz, Sebastian;Watz, Henrik
前言：慢性阻塞性肺疾病(COPD)以中性粒细胞炎症为特征。中性粒细胞细胞外陷阱(Net)的形成--一个由中性粒细胞DNA成分和中性粒细胞酶组成的网络，参与了先天免疫和炎症。目前对缓解期COPD患者诱导痰中是否存在上述结构知之甚少。方法：收集23例COPD患者和10例健康对照的诱导痰标本。收集痰细胞，培养，染色网状成分。用NanoDrop 2000分光光度计定量细胞外DNA。结果：与健康对照组相比，COPD痰中Net的形成明显上调，无论是化脓性痰还是吸烟状态。净形成与痰上清液中细胞外DNA浓度显著升高有关(慢性阻塞性肺病组L 484 ng/亩，对照组L 268 ng/亩，p=0.013)。对数转化的细胞外DNA与对数转化的中性粒细胞绝对数(r=0.6；p<；0.001)和呼吸道阻塞(r=-0.43；p=0.013)相关。结论：细胞外DNA浓度较高的网状形成可能是慢性阻塞性肺疾病痰中性粒细胞的病理生物学特征。(C)2015爱思唯尔有限公司。保留所有权利。
Introduction: Chronic obstructive pulmonary disease (COPD) is characterized by neutrophilic airway inflammation. Neutrophil extracellular trap (NET) formation - a meshwork of neutrophil DNA components and neutrophil enzymes are involved in innate immunity and inflammation. Little is known about the presence of these structures in induced sputum from stable COPD patients.Methods: Induced sputum samples of 23 COPD patients and 10 healthy controls were collected. Sputum cells were harvested, cultivated and stained for NET components. Extracellular DNA was quantified using a NanoDrop 2000 spectrophotometer.Results: NET formation was markedly upregulated in COPD sputum compared with healthy controls, irrespective of sputum purulence or smoking status. NET formation was associated with significantly higher concentration of extracellular DNA in sputum supernatant (484 ng/mu l in COPD versus 268 ng/mu l in controls, p = 0.013). Log-transformed extracellular DNA correlated with log-transformed absolute neutrophil numbers in sputum (r = 0.60; p < 0.001) and airway obstruction (r = -0.43; p = 0.013).Conclusion: NET formation associated with higher concentrations of extracellular DNA may be a pathobiological feature of COPD-derived sputum neutrophils. (C) 2015 Elsevier Ltd. All rights reserved.