Fibronectin pattern in benign hyperplasia and cancer of the prostate
Fibronectin pattern in benign hyperplasia and cancer of the prostate
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DOI：
10.1155/2008/308420
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Kosanovic, Maja M.
中科院分区：
文献类型：
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作者：
Jankovic, Miroslava M.;Kosanovic, Maja M.
纤维连接蛋白（FN）是一种参与细胞-基质相互作用的多功能糖蛋白。它在氨基酸和低聚糖组成方面表现出不同形式的复杂模式。在这项研究中，我们检测了FN在良性前列腺增生（BPH）和前列腺癌（PCa）中的糖生化和功能特性，试图解决疾病相关的差异。2个BPH血清池和3个PCa血清池作为FN来源。通过SDS-PAGE、免疫和凝集素印迹、凝集素亲和层析和粘附实验对亲和纯化的分子进行了表征。BPH FN作为完整分子存在，在220 kDa处形成主要免疫反应带。相比之下，PCa FN包含三个主要的免疫反应片段，分别为140、110和90 kDa。在糖基化方面，总凝集素活性PCa FN与BPH FN的比例不同，表现为Con a -的减少和lca -的增加。成纤维细胞对两种FN的粘附均呈浓度依赖性，但对PCa FN的粘附效率明显较低。所获得的结果显示，与BPH FN相比，PCa FN具有明显的结构特征，这可能对其配体的调节和识别特性很重要，这些特性表现为功能的获得或丧失，或作为其起源的特定标记。
Fibronectin (FN) is a multifunctional glycoprotein involved in cell-matrix interactions. It exhibits a complex pattern of forms differing in respect to aminoacid and oligosaccharide composition. In this study we examined glycobiochemical and functional properties of the FN in benign prostatic hyperplasia (BPH) and prostatic cancer (PCa), attempting to resolve disease-related differences. Two BPH sera pools and three PCa sera pools were used as the FN source. The affinity-purified molecule was characterized by SDS-PAGE, immuno- and lectin blot, lectin-affinity chromatography and adhesion assay. BPH FN existed as intact molecule, giving the main immunoreactive band at 220 kDa. In contrast, PCa FN comprised three main immunoreactive fragments of 140, 110 and 90 kDa. As for glycosylation the ratio of altogether lectin-reactive PCa FN was different from that of BPH FN manifested as a decrease of Con A- and an increase of LCA-reactive moieties. Fibroblasts adhered to both FN preparations in a concentration dependent manner, but with a significantly lower efficiency to PCa FN. The results obtained showing distinct structural characteristics of PCa FN compared to BPH FN could be important for modulation of its ligand and recognition properties expressed as gain or loss of functions or as specific markers of its origin.