Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination.
Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination.
复制标题
转座子 Tn3 编码位点特异性重组系统：识别必需序列、基因和实际重组位点。
DOI：
10.1073/pnas.78.7.4041
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Heffron,F
中科院分区：
文献类型：
--
作者：
Kostriken,R;Morita,C;Heffron,F
细菌转座子Tn3编码一种定点重组系统。这种重组需要tnpR的产物，tnpR是一种以前被认为是转座酶抑制因子的基因。这种重组是位点特异性的，发生在序列C-G-A-T-A-T-T-A-T-A-T-T-A-T-C内，但需要至少一个额外的序列。这种重组系统中突变的表型表明，转座是通过一种机制进行的，其中共整合是中间产物。
The bacterial transposon Tn3 encodes a site-specific recombination system. The recombination requires the product of tnpR, a gene previously identified as a repressor of the transposase. This recombination is site specific and takes place somewhere within the sequence C-G-A-A-A-T-A-T-T-A-T-A-A-A-T-T-A-T-C but requires at least one additional sequence outside this. The phenotype of mutations in this recombination system suggests that transposition proceeds by a mechanism in which cointegrates are intermediates.