Inhibitory and excitatory neurotransmitters in the cerebrospinal fluid of epileptic dogs
Inhibitory and excitatory neurotransmitters in the cerebrospinal fluid of epileptic dogs
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DOI：
10.2460/ajvr.2004.65.1108
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发表时间：
2004-08-01
影响因子：
1
通讯作者：
Jaggy, A
中科院分区：
文献类型：
--
作者：
Ellenberger, C;Mevissen, M;Jaggy, A
目的-测定大量患有特发性癫痫或遗传性癫痫的犬的CSF中兴奋性和抑制性氨基酸的浓度，并评价CSF中氨基酸浓度与药物治疗和性别有关的变化。（20只雄性和15只雌性），94只患有特发性癫痫的非拉布拉多猎犬（71只雄性和23只雌性）和20只对照犬（10只雄性和10只雌性）。脑脊液中γ-氨基丁酸（GABA）、谷氨酸（GLU）、天冬氨酸（ASP）、丝氨酸，和甘氨酸测定使用高效液相色谱电化学detect.Results-CSF中的GABA和GLU浓度显着低于拉布拉多猎犬与遗传性癫痫（LR组狗）比对照组狗或非拉布拉多猎犬与特发性癫痫（非LR组狗）。LR组犬的Glu与GABA比值显著高于非LR组犬。CSF浓度的GLU和ASP显着降低时，所有的狗与癫痫（非LR-和LR-组狗相结合）与对照组dogs.Conclusions和临床相关性GABA的CSF浓度的减少似乎发挥作用的发病机制中的遗传决定的癫痫拉布拉多猎犬。然而，脑脊液中GABA浓度的降低也可能是癫痫发作活动的结果。Glu与GABA的比值可能被证明是拉布拉多猎犬遗传性癫痫的一个有用指标。
Objective-To determine concentrations of excitatory and inhibitory amino acids in CSF of a large number of dogs with idiopathic epilepsy or genetic epilepsy and to evaluate changes in CSF amino acid concentration with regard to drug treatment and sex.Animals-35 Labrador Retrievers with genetic epilepsy (20 male and 15 female), 94 non-Labrador Retrievers with idiopathic epilepsy (71 male and 23 female), and 20 control dogs (10 male and 10 female).Procedure-Collection of CSF was performed >72 hours after the occurrence of seizures. Cerebrospinal fluid concentrations of gamma-aminobutyric acid (GABA), glutamate (GLU), aspartate (ASP), serine, and glycine were determined by use of high performance liquid chromatography with electrochemical detection.Results-CSF concentrations of GABA and GLU were significantly lower in Labrador Retrievers with genetic epilepsy (LR-group dogs) than in control-group dogs or in non-Labrador Retrievers with idiopathic epilepsy (non-LR-group dogs). The GLU-to-GABA ratio was significantly higher in LR-group dogs than in non-LR-group dogs. CSF concentrations of GLU and ASP were significantly lower when all dogs with epilepsy (non-LR- and LR-group dogs combined) were compared with control-group dogs.Conclusions and Clinical Relevance A decrease in CSF concentrations of GABA appears to play a role in the pathogenesis of genetically determined epilepsy in Labrador Retrievers. However, this decrease in CSF concentrations of GABA may also be a consequence of seizure activity. The GLU-to-GABA ratio may prove to be a useful indicator of genetic epilepsy in Labrador Retrievers.