Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa
Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa
复制标题
DOI：
10.7554/elife.47365
复制
发表时间：
2019-11-12
期刊：
影响因子：
7.7
通讯作者：
O'Toole, George A.
中科院分区：
文献类型：
--
作者：
Limoli, Dominique H.;Warren, Elizabeth A.;O'Toole, George A.
微生物通常生活在多物种群落中，群落成员之间的相互作用会影响个体成分和周围环境。在这里，我们开发了一个系统来可视化物种间的行为在最初的遭遇。通过成像已知从慢性疾病中共同分离的两种流行病原体，铜绿假单胞菌和金黄色葡萄球菌，我们观察到铜绿假单胞菌可以响应于S.金黄色。在感测到S.金黄色葡萄球菌、铜绿假单胞菌从集体运动转变为单细胞运动，并伴随着速度和方向性的增加--我们将这种行为称为“探索运动”。Explorer细胞优先向S移动。aureus感染S.通过IV型皮利的作用产生金黄色菌落。这些研究揭示了以前未描述的运动行为，并深入了解铜绿假单胞菌如何感知和响应其他物种。确定利用这些相互作用的策略可能为新的抗菌策略开辟道路。
Microbes often live in multispecies communities where interactions among community members impact both the individual constituents and the surrounding environment. Here, we developed a system to visualize interspecies behaviors at initial encounters. By imaging two prevalent pathogens known to be coisolated from chronic illnesses, Pseudomonas aeruginosa and Staphylococcus aureus, we observed P. aeruginosa can modify surface motility in response to secreted factors from S. aureus. Upon sensing S. aureus, P. aeruginosa transitioned from collective to single-cell motility with an associated increase in speed and directedness - a behavior we refer to as 'exploratory motility'. Explorer cells moved preferentially towards S. aureus and invaded S. aureus colonies through the action of the type IV pili. These studies reveal previously undescribed motility behaviors and lend insight into how P. aeruginosa senses and responds to other species. Identifying strategies to harness these interactions may open avenues for new antimicrobial strategies.