The activity-regulated cytoskeleton-associated (arc) gene is a new light-inducible early gene in the mouse suprachiasmatic nucleus
The activity-regulated cytoskeleton-associated (arc) gene is a new light-inducible early gene in the mouse suprachiasmatic nucleus
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DOI：
10.1016/s0306-4522(02)00786-8
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发表时间：
2003-02
期刊：
影响因子：
3.3
通讯作者：
M. Nishimura;K. Yamagata;H. Sugiura;H. Okamura
中科院分区：
文献类型：
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作者：
M. Nishimura;K. Yamagata;H. Sugiura;H. Okamura
哺乳动物的昼夜节律系统是通过改变视交叉上核中主时钟的相位而被带入环境光/暗周期的。伴随着光诱导的相移，各种立即早期基因出现在视交叉上核时钟细胞，在这里，我们报告了一个新的立即早期基因弧（活性调节的细胞因子相关基因）在小鼠中的表达。Arc信使RNA在主观夜间（CT 12-CT 20）光照后30-120 min在视交叉上核视网膜受体区神经元中被强烈诱导，尽管它们的自发表达在通常的光暗周期和恒定的黑暗条件下不存在。在蛋白质水平上，ARC不仅出现在视交叉上核神经元的核内，而且还出现在视交叉上核神经元的胞体及其突起内。这些发现表明，Arc是一个活性调节的细胞骨架基因，可能参与光诱导的昼夜节律相移。
The mammalian circadian system is entrained to the environmental light/dark cycle by shifting the phase of the master clock in the suprachiasmatic nucleus. Accompanying the light-induced phase-shift, a variety of immediate-early genes appears in suprachiasmatic nucleus clock cells, and here, we report the expression of a new immediate-early gene Arc (activity-regulated cytoskelton-associated gene) in mice. Arc messenger RNAs were strongly induced at 30-120 min after the light exposure at subjective night (CT12-CT20) in neurons of the retinorecipient area of the suprachiasmatic nucleus, although their spontaneous expression was absent in usual light-dark cycles and in constant dark conditions. At protein level, ARC appeared not only in the nucleus but also in the perikarya and their processes of the suprachiasmatic nucleus neurons. These findings indicate that Arc is an activity-regulated cytoskeletal gene possibly involved in the light-induced phase-shift of the circadian rhythm.