Cloning and functional characterization of three novel antimicrobial peptides from tilapia (Oreochromis niloticus)
Cloning and functional characterization of three novel antimicrobial peptides from tilapia (Oreochromis niloticus)
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DOI：
10.1016/j.aquaculture.2012.07.032
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发表时间：
2013-01-24
期刊：
影响因子：
4.5
通讯作者：
Pablo Estrada, Mario
中科院分区：
文献类型：
--
作者：
Acosta, Jannel;Montero, Vivian;Pablo Estrada, Mario
抗菌肽是先天免疫系统的重要组成部分。硬骨鱼是发现新型抗菌肽的潜在丰富资源，因为这些生物体显著依赖其先天免疫系统来对抗水生环境中感染的持续威胁。在本研究中，我们从罗非鱼（Oreochromis niloticus）鳃的EST报告序列的基础上分离的抗菌肽类转录本。这些肽被命名为Oreochromicins（Oreoch-1、Oreoch-2和Oreoch-3）。这些推定的AMP的cDNA序列编码与硬骨鱼的鱼杀菌素家族的成员具有最高相似性的三个前原肽。预测的三个前原肽由信号肽、分别为23、25和32个氨基酸的高度阳离子成熟肽和羧基末端前结构域组成。合成肽对革兰氏阴性菌、革兰氏阳性菌和真菌具有广谱抗菌活性。这些肽在脑、心脏、头肾、脾和肠中组成型表达。此外，他们的结合特性，脂多糖和细胞毒活性在哺乳动物和鱼类细胞进行了测定。(C)2012年爱思唯尔B。V.保留所有权利。
Antimicrobial peptides constitute an important component of the innate immune system. Teleost fish represent a potentially fruitful resource for novel antimicrobial peptide discovery since these organisms rely significantly on their innate immune systems to combat the constant threat of infections in the aquatic environment. In the present study, we isolated three antimicrobial peptides-like transcripts from tilapia (Oreochromis niloticus) gills based on EST reported sequences. These peptides were named oreochromicins (Oreoch-1, Oreoch-2 and Oreoch-3). The cDNA sequences for these putative AMPs encode three pre-pro-peptides with the highest similarity with the members of the piscidin family from teleost fish. The predicted three pre-pro-peptides consist of a signal peptide, a highly cationic mature peptide of 23, 25, and 32 amino acids, respectively and a carboxy terminal pro-domain. The synthetic peptides displayed a broad-spectrum of antimicrobial activity against Gram-negative, Gram-positive bacteria and fungi. These peptides are constitutively expressed in the brain, heart, head kidney, spleen and gut. Additionally, their binding properties to lipopolysaccharide and cytotoxic activity in mammalian and fish cells were assayed. (C) 2012 Elsevier B. V. All rights reserved.