AN ALTERNATIVELY SPLICED FORM OF THE HUMAN LYSOSOME-ASSOCIATED MEMBRANE PROTEIN-2 GENE IS EXPRESSED IN A TISSUE-SPECIFIC MANNER
AN ALTERNATIVELY SPLICED FORM OF THE HUMAN LYSOSOME-ASSOCIATED MEMBRANE PROTEIN-2 GENE IS EXPRESSED IN A TISSUE-SPECIFIC MANNER
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DOI：
10.1006/bbrc.1995.2528
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发表时间：
1995-10-13
影响因子：
3.1
通讯作者：
KONECKI, UL
中科院分区：
文献类型：
--
作者：
KONECKI, DS;FOETISCH, K;KONECKI, UL
我们报道了在人肌肉中过表达的选择性剪接的人溶酶体相关膜蛋白2 （h-lamp-2）转录物的分离。该转录物的克隆表明了溶酶体膜蛋白的组织特异性表达，并暗示了h-lamp-2基因的蛋白产物以及其他溶酶体相关膜蛋白的多种功能的可能性。这个新的转录本被命名为h-lamp-2b，它是由最后一个外显子（外显子9）的选择性剪接产生的，其选择性剪接长度约为2800 bp。所得蛋白与先前报道的h-lamp-2蛋白长度相同，包括前导肽在内的410个氨基酸。最后的外显子编码腔域的最后11个氨基酸，24个氨基酸跨膜区和11个氨基酸的细胞质尾部，显示了Gly的完整保存。酪氨酸。X。X溶酶体靶向信号相对于跨膜区和蛋白质的羧基末端的位置。免疫电镜研究证实了这种替代基因产物在溶酶体膜上的定位。(C) 1995学术出版社，Inc。
We report the isolation of an alternatively spliced human lysosome-associated membrane protein-2 (h-lamp-2) transcript which is overexpressed in human muscle. The cloning of this transcript is an indication for the tissue-specific expression of lysosomal membrane proteins and implicates the possibility of multiple functions for the protein products of the h-lamp-2 gene, as well as other lysosome-associated membrane proteins. The new transcript, designated h-lamp-2b, results from the alternative splicing of the last exon, exon 9, the alternative form of which is approximately 2800 bp in length. The resulting protein is identical in length to the previously reported h-lamp-2 protein, 410 amino acids including the leader peptide. This final exon, which encodes the last eleven amino acids of the luminal domain, the 24 amino acid transmembrane spanning region, and an eleven amino acid cytoplasmic tail, shows complete conservation of the Gly . Tyr . X . X lysosomal targeting signal with regard to its position relative to the transmembrane spanning region and the carboxy terminus of the protein. Immune electron microscopy studies verified localization of this alternative gene product to the lysosomal membrane. (C) 1995 Academic Press, Inc.