Seven-up controls switching of transcription factors that specify temporal identities of Drosophila neuroblasts
Seven-up controls switching of transcription factors that specify temporal identities of Drosophila neuroblasts
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DOI：
10.1016/j.devcel.2004.12.014
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发表时间：
2005-02-01
影响因子：
11.8
通讯作者：
Hiromi, Y
中科院分区：
文献类型：
--
作者：
Kanai, MI;Okabe, M;Hiromi, Y
果蝇神经干细胞（NB）在分裂时不断改变特征，在下一次分裂时产生不同类型的后代。转录因子Hunchback（HB）、Kruppel（KR）、Pdm（PDM）等在每个NB中顺序表达，并作为出生顺序身份的决定因素。NB如何将其表达谱从一种转录因子转换到另一种转录因子还知之甚少。我们表明，HB-KR开关是由核受体七（SVP）。SVP表达局限于NB谱系内的时间限制的子部分。SVP功能的丧失导致几个NB谱系内HB阳性细胞数量的增加，而svp的错误表达导致这些早期出生的神经元的丧失。血统分析提供的证据表明，需要svp在适当的时候关闭HB。因此，svp修改了自我更新的干细胞程序，允许细胞命运的时间顺序变化，从而产生神经元的多样性。
Drosophila neuronal stem cell neuroblasts (NB) constantly change character upon division, to produce a different type of progeny at the next division. Transcription factors Hunchback (HB), Kruppel (KR), Pdm (PDM), etc. are expressed sequentially in each NB and act as determinants of birth-order identity. How a NB switches its expression profile from one transcription factor to the next is poorly understood. We show that the HB-to-KR switch is directed by the nuclear receptor Seven-up (SVP). SVP expression is confined to a temporally restricted subsection within the NB's lineage. Loss of SVP function causes an increase in the number of HB-positive cells within several NB lineages, whereas misexpression of svp leads to the loss of these early-born neurons. Lineage analysis provides evidence that svp is required to switch off HB at the proper time. Thus, svp modifies the self-renewal stem cell program to allow chronological change of cell fates, thereby generating neuronal diversity.