Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility
Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility
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DOI：
10.1139/b09-085
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发表时间：
2010-03-01
期刊：
影响因子：
1.1
通讯作者：
Sawhney, Vipen K.
中科院分区：
文献类型：
--
作者：
Sheoran, Inder S.;Sawhney, Vipen K.
在甘蓝型油菜Ogura(OGU)细胞质雄性不育(CMS)系统中，雄性不育是由线粒体基因缺陷引起的，在我们的生长条件下，产生的雄蕊为心型。我们分析了OGU和正常花药的蛋白质组，目的是识别差异表达的蛋白质及其在花粉发育和雄性不育中的潜在作用。利用差示凝胶内电泳法，我们在正常花药和OGU花药的双向凝胶上检测到2300多个点，在167个2倍或更高表达的点中，用MALDI-TOF/TOF质谱仪分析了120个点，通过NCBI和Brassica EST数据库搜索，鉴定了94个蛋白质。在正常花药中上调的蛋白质包括与碳水化合物和能量代谢相关的蛋白质(如果糖二磷酸酶、三磷酸腺苷合成酶、苹果酸脱氢酶和细胞色素c氧化酶)、细胞壁重塑(如β-1，3-葡聚糖酶和果胶酯酶)、乙醛脱氢酶(ALDH)、光合作用和类黄酮合成。在正常花药和OGU花药中都检测到了蛋白水解酶，但OGU花药中一些蛋白水解酶抑制剂表达上调。正常花药的ALDH和β-1，3-葡聚糖酶活性也显著高于普通花药。本研究证明了线粒体-核相互作用在甘蓝型油菜OGU细胞质雄性不育控制中的蛋白质组学基础。
In the Ogura (ogu) cytoplasmic male sterility (CMS) system in Brassica napus L., male sterility is caused by a defect in the mitochondrial gene and, under our growth conditions, stamens produced are carpelloid. We analyzed the proteome of ogu and normal anthers with the objective of identifying differentially expressed proteins and their potential roles in pollen development and male sterility. By using differential in-gel electrophoresis, we detected over 2300 spots on 2-D gels of normal and ogu anthers and of the 167 spots with 2-fold or higher expression, 120 were analyzed by MALDI-TOF/TOF mass spectrometry and 94 proteins were identified searching against NCBI and Brassica EST databases. Proteins up-regulated in normal anthers included those associated with carbohydrate and energy metabolism (e.g., fructose bisphosphatase, ATP synthase, malate dehydrogenase, and cytochrome c oxidase), cell wall remodeling (e.g., beta-1,3-glucanase and pectinesterase), aldehyde dehydrogenase (ALDH), photosynthesis, and flavonoid synthesis. Proteases were identified in both the normal and ogu anthers, but a number of protease inhibitors were up-regulated in ogu anthers. The activities of ALDH and beta-1,3-glucanase were also much higher in normal anthers by comparison with ogu anthers. this study documents the proteomic basis of mitochondrial-nuclear interaction in the control of ogu CMS in B. napus.