Fiber Type-Specific Expression of Low-Density Lipoprotein Receptor-Related Protein 6 in Human Skeletal Muscles
Fiber Type-Specific Expression of Low-Density Lipoprotein Receptor-Related Protein 6 in Human Skeletal Muscles
复制标题
人骨骼肌中低密度脂蛋白受体相关蛋白 6 的纤维类型特异性表达
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
M. Matsumoto
中科院分区：
文献类型：
--
作者：
I. Takeda;Tetsuya Takahashi;K. Ochi;T. Kurashige;Yukari Shinozaki;M. Nakamori;K. Arihiro;H. Maruyama;M. Matsumoto
目的：两种骨骼肌纤维的基因表达模式不同。Wnt信号通路，包括低密度脂蛋白受体相关蛋白6 (LRP6)，与骨骼肌细胞分化和葡萄糖代谢有关。我们检测了肌纤维类型与LRP6表达之间的关系。方法：采用组织化学方法检测16例肌肉组织切片中腺苷三磷酸酶（Adenosine triphosphatase）的表达，免疫组织化学方法分析LRP6和肌球蛋白的表达水平。免疫细胞化学检测LRP6在C2C12细胞中的表达谱。结果：LRP6仅在II型纤维中表达。IIc型纤维中LRP6表达存在差异。LRP6在成肌细胞分化阶段表达。结论：LRP6抗体可用于ⅱ型骨骼肌纤维的鉴别。LRP6可能影响人类骨骼肌II型纤维的葡萄糖代谢。
Objective: Gene expression patterns differ in the two types of skeletal muscle fiber. The Wnt signaling pathway, which includes low-density lipoprotein receptor-related protein 6 (LRP6), has been associated with cell differentiation and glucose metabolism in skeletal muscles. We examined the relationships between muscle fiber types and LRP6 expression. Methods: Adenosine triphosphatase was assayed histochemically, and the levels of expression of LRP6 and myosin were analyzed immunohistochemically, in frozen sections of muscle fiber obtained from 16 muscle biopsy samples. The expression pattern of LRP6 in C2C12 cells was assayed by immunocytochemistry. Results: LRP6 was expressed only in type II fibers. Type IIc fibers showed variations in LRP6 expression. Expression of LRP6 was observed at the stage of myoblast differentiation. Conclusion: Antibody to LRP6 may be useful for identifying type II skeletal muscle fibers. LRP6 may influence glucose metabolism in type II fibers of human skeletal muscles.
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影响因子：
10.5
作者：
Chin, ER;Olson, EN;Williams, RS
通讯作者：
Williams, RS
影响因子：
6
作者：
Pevzner, Alexandra;Schoser, Benedikt;Kroeger, Stephan
通讯作者：
Kroeger, Stephan
影响因子：
3
作者：
Horton, MJ;Brandon, CA;Sciote, JJ
通讯作者：
Sciote, JJ
影响因子：
29
作者：
Singh R;De Aguiar RB;Naik S;Mani S;Ostadsharif K;Wencker D;Sotoudeh M;Malekzadeh R;Sherwin RS;Mani A
通讯作者：
Mani A
影响因子：
11.2
作者：
Tahir SA;Yang G;Goltsov A;Song KD;Ren C;Wang J;Chang W;Thompson TC
通讯作者：
Thompson TC