Characterization of the role played by the RAD59 gene of Saccharomyces cerevisiae in ectopic recombination
Characterization of the role played by the RAD59 gene of Saccharomyces cerevisiae in ectopic recombination
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DOI：
10.1007/s002940050467
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发表时间：
1999-08-01
期刊：
影响因子：
2.5
通讯作者：
Kupiec, M
中科院分区：
文献类型：
--
作者：
Jablonovich, Z;Liefshitz, B;Kupiec, M
酵母中的RAD52基因组通过重组机制控制DNA损伤的修复。尽管越来越多的证据表明修复组蛋白之间的物理和生化相互作用，但个体基因的突变对不同类型的重组表现出非常不同的影响。RAD59基因编码一种与Rad52p相似的蛋白，该蛋白在电离辐射引起的损伤修复中起作用。在本研究中，我们研究了Rad59蛋白在有丝分裂异位重组中的作用，并分析了与修复组其他成员的遗传相互作用。我们发现Rad59p在异位基因转换中发挥作用，这种作用依赖于Rad52p的存在，但独立于RecA同源物Rad51p和Rad57蛋白的功能。RAD59基因产物也参与rad1依赖的直接重复序列之间的重组途径。我们认为Rad59p可能在Rad51丝不起作用时起救助机制的作用。
The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types of recombination. The RAD59 gene encodes a protein with similarity to Rad52p which plays a role in the repair of damage caused by ionizing radiation. In the present study we have examined the role played by the Rad59 protein in mitotic ectopic recombination and analyzed the genetic interactions with other members of the repair group. We found that Rad59p plays a role in ectopic gene conversion that depends on the presence of Rad52p but is independent of the function of the RecA homologue Rad51p and of the Rad57 protein. The RAD59 gene product also participates in the RAD1-dependent pathway of recombination between direct repeats. We propose that Rad59p may act in a salvage mechanism that operates when the Rad51 filament is not functional.