Protective effects of a protein-bound polysaccharide, PSK, on Candida albicans infection in mice via tumor necrosis factor-α induction
Protective effects of a protein-bound polysaccharide, PSK, on Candida albicans infection in mice via tumor necrosis factor-α induction
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DOI：
10.1016/s1567-5769(01)00104-7
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发表时间：
2001-09-01
影响因子：
5.6
通讯作者：
Ando, T
中科院分区：
文献类型：
--
作者：
Ohmura, Y;Matsunaga, K;Ando, T
我们研究了蛋白质结合多糖 PSK 对小鼠白色念珠菌（C. albicans）致死性感染的保护机制。 (1) 在静脉注射白色念珠菌的 BALB/c 小鼠中，腹膜内 (ip) 施用 PSK 可以提高存活率并延长存活期，具体取决于施用时间、剂量和真菌接种量；当在接种1×10(6)白色念珠菌前24小时向小鼠腹膜内施用PSK 250 mg/kg时，获得最大效果（30天存活率显示为60%，致命感染小鼠的平均存活期增加209%）。 (2)经环磷酰胺或角叉菜胶处理的小鼠，或先前经抗肿瘤坏死因子-α(TNF-α)抗体处理的小鼠，PSK的保护作用显着降低。 (3)接种后6小时至第1天，给予PSK可显着增强脾脏TNF-α基因的表达，并增强白细胞功能。 (4)当使用经过ss1-3葡聚糖酶或肼水解的PSK级分代替PSK时，抗真菌活性显着降低。这些结果表明，PSK对小鼠致死性白色念珠菌感染的保护作用主要是通过TNF-α功能产生的，并且PSK分子中的ss1-3葡聚糖和蛋白质部分参与了该活性的表达。 (C) 2001 Elsevier Science B.V. 保留所有权利。
We investigated the protective mechanism of a protein-bound polysaccharide, PSK, against lethal infection with Candida albicans (C. albicans) in mice. (1) In BALB/c mice inoculated intravenously with C albicans, the intraperitoneal (ip) administration of PSK increased survival rates and prolonged the survival period depending on the time of administration, the dosage, and the size of fungal inoculum; the maximal effect was obtained when PSK 250 mg/kg was ip administered to mice 24 h before inoculation of I x 10(6) C. albicans (30 days survivors showed 60% and the mean survival period of mice with fatal infection increased 209%). (2) The protective effect of PSK was significantly decreased in mice treated with cyclophosphamide or carrageenan, or in mice treated previously with anti-tumor necrosis factor-alpha (TNF-alpha) antibody. (3) The administration of PSK significantly enhanced the expression of TNF-alpha gene in spleen and increased leukocyte functions from 6 h to I day after inoculation. (4) When the PSK fraction subjected to hydrolysis with ss1-3 glucanase or hydrazine was used instead of PSK, the anti-fungal activities were significantly decreased. These findings suggested that the protective effect of PSK on lethal C. albicans infection in mice was mainly produced via TNF-alpha functions, and that ss1-3 glucan and protein moiety in PSK molecule were involved in the expression of the activities. (C) 2001 Elsevier Science B.V. All rights reserved.