Fluoro-Jade B stains quiescent and reactive astrocytes in the rodent spinal cord
Fluoro-Jade B stains quiescent and reactive astrocytes in the rodent spinal cord
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DOI：
10.1089/089771503770802899
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发表时间：
2003-11-01
影响因子：
4.2
通讯作者：
Scheff, SW
中科院分区：
文献类型：
--
作者：
Anderson, KJ;Fugaccia, I;Scheff, SW
为了标记受伤脊髓中死亡的神经元，我们使用了新型荧光素衍生物Fluoro-Jade B，据报道它可以特异性标记大脑中死亡或死亡的神经元。使用IH撞击装置对大鼠和小鼠进行中度脊髓损伤，并在损伤后1、2、4、7、14和21天处死。脊髓组织进行氟玉B组织化学处理，包括整个脊髓损伤区域的切片。损伤后任何时间点脊髓任何水平的切片均未观察到氟玉阳性神经元。相反，氟玉标记的星形胶质细胞在未受伤的对照动物和受伤的动物。氟玉蛋白与胶质纤维酸性蛋白共定位证实了星形细胞染色的特异性。我们还对一组大鼠进行了连续的皮质挫伤和脊髓损伤。这些动物的切片显示皮层挫伤下的海马和丘脑中有大量氟玉阳性神经元；然而，脊髓的染色模式与那些单独接受脊髓损伤的动物是相同的。
In an attempt to label dying neurons in the injured spinal cord, we used the novel fluorescein derivative Fluoro-Jade B, which has been reported to specifically label dead or dying neurons in the brain. Rats and mice were subjected to a moderate level of spinal cord injury using an IH impact device and sacrificed at 1, 2, 4, 7, 14, and 21 days post injury. Spinal cord tissue was processed for Fluoro-Jade B histochemistry and included sections throughout the injured region of the cord. No Fluoro-Jade positive neurons were observed in sections from any time point postinjury at any level of the spinal cord. Instead, Fluoro-Jade labeled astrocytes in uninjured control animals and injured animals. The specificity of astrocytic staining was confirmed by co-localizaton of Fluoro-Jade with glial fibrillary acidic protein. We also subjected a group of rats to a sequential cortical contusion injury and spinal cord injury. Sections from these animals showed numerous Fluoro-Jade positive neurons in the hippocampal formation and thalamus underlying the cortical contusion; however, the staining pattern in the spinal cord was identical to those animals that had received spinal cord injury alone.