Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers
Genome-wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers
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DOI：
10.1111/j.1471-8286.2007.01826.x
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发表时间：
2007-11-01
期刊：
影响因子：
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通讯作者：
Wade, Michael J.
中科院分区：
文献类型：
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作者：
Demuth, Jeffery P.;Drury, Douglas W.;Wade, Michael J.
对赤拟谷盗全基因组序列进行了1-6 bp的微卫星序列分析。从12000多个微卫星中筛选出981对引物，得到509个多态性标记。我们报告的多态性数据汇总DNA样本从3至11个地理分布广泛的T。castaneum种群以及标记子集的杂合性。微卫星的组成和染色体分布的简要讨论与以前的研究。
We survey the Tribolium castaneum whole genome sequence for 1-6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies.