The HIV-1 transgenic rat as a model for HIV-1 infected individuals on HAART
The HIV-1 transgenic rat as a model for HIV-1 infected individuals on HAART
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DOI：
10.1016/j.jneuroim.2009.09.014
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发表时间：
2010-01-25
影响因子：
3.3
通讯作者：
Chang, Sulie L.
中科院分区：
文献类型：
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作者：
Peng, Jinsong;Vigorito, Michael;Chang, Sulie L.
在接受高效抗逆转录病毒治疗（HAART）的患者中，HIV-1病毒复制有限：然而，HIV-1病毒蛋白仍然存在。我们证明，发展中的HIV-1 Tg大鼠，表达所有的HIV-1病毒基因，除了gag-pol复制基因，保持较低的体重与F344对照大鼠相比。尽管HIV-1 Tg大鼠的饮食比对照组动物少，但它们并不饥饿，也没有表现出快感缺乏的迹象。在19月龄时，HIV-1 Tg大鼠开始表现出消瘦的临床体征，并逐渐死亡。使用实时RT-PCR，我们比较了2-3月龄与10-11月龄的HIV-1 Tg大鼠中HIV病毒蛋白达特、gp 120、nef和vif的表达。幼龄大鼠脾脏病毒蛋白RNA水平显著高于老龄大鼠（P
HIV-1 viral replication is limited in patients given highly active anti-retroviral therapy (HAART): however, HIV-1 viral proteins are still present. We demonstrate that the developing HIV-1Tg rat, which expresses all of the HIV-1 viral genes except the gag-pol replication genes, maintains lower body weight compared with the F344 control rat. Although HIV-1Tg rats eat and drink less than the control animals, they are not anorexic and show no evidence of anhedonia. At 19 months (mo) of age, HIV-1Tg rats begin to show clinical signs of wasting that progress to death. Using real-time RT-PCR, we compared the expression of the HIV viral proteins Tat, gp120, nef, and vif, in the HIV-1Tg rats at 2-3 mo of age with those at 10-11 mo of age. RNA levels of viral protein in the spleens of younger rats were significantly greater than those in the older rats (P