Estrogen receptor analysis by flow cytometry.
Estrogen receptor analysis by flow cytometry.
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通过流式细胞术进行雌激素受体分析。
DOI：
10.1126/science.6719116
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Barlogie,B
中科院分区：
文献类型：
--
作者：
Van,NT;Raber,M;Barrows,GH;Barlogie,B
用荧光标记的雌二醇-N′-荧光素基-N ′-（17β-雌二醇半琥珀酰胺）硫脲（FE）测定肿瘤细胞中雌激素受体的含量。用流式细胞仪定量测定FE的细胞含量。FE的结合发生在纳摩尔浓度范围内，这表明标记的雌二醇具有高亲和力。FE与雌激素竞争结合位点，但不与孕激素、雄激素或糖皮质激素竞争，表明FE结合的特异性。与其他雌激素受体测定法相比，这种新技术需要小样本量（约5000个细胞），并允许评估肿瘤细胞中雌激素受体表达的异质性。
A fluorescently labeled estradiol, N′-fluoresceino-N′-(17β-estradiol hemisuccinamide) thiourea (FE) was used for measuring estrogen receptor content per cell in tumor cells. The cellular content of FE was measured quantitatively by flow cytometry. Binding of FE occurs in the nanomolar concentration range, an indication of the high affinity of the labeled estradiol. Competition of FE for binding sites is observed with estrogens, but not with progestins, androgens, or glucocorticosteroids, indicating the specificity of FE binding. In contrast to other estrogen receptor assays, this new technique requires a small sample size (about 5000 cells) and permits the assessment of heterogeneity in estrogen receptor expression among tumor cells.