ROTAVIRUS ISOLATE WI61 REPRESENTING A PRESUMPTIVE NEW HUMAN SEROTYPE
ROTAVIRUS ISOLATE WI61 REPRESENTING A PRESUMPTIVE NEW HUMAN SEROTYPE
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DOI：
10.1128/jcm.25.9.1757-1762.1987
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发表时间：
1987-09-01
影响因子：
9.4
通讯作者：
OFFIT, PA
中科院分区：
文献类型：
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作者：
CLARK, HF;HOSHINO, Y;OFFIT, PA
1983年2月，从费城儿童医院（美国宾夕法尼亚州）收治的1例18个月大胃肠炎患儿中分离出一种代表推定新人类血清型的病毒（毒株WI 61）。通过交叉中和试验，WI 61病毒与血清型1、2、3和4的人轮状病毒、人69 M以及代表性牛（NCDV）、猪（OSU）和鸡（Ch2）轮状病毒明确区分开来。在对WI 61病毒超免疫的豚鼠中产生的抗血清显示与人血清型1、2、3和4的轮状病毒的部分交叉反应性。通过对相关轮状病毒的研究，推测确定WI 61病毒交叉反应性抗原决定簇位于由基因片段4编码的vp3表面多肽上。在1983年和1984年发现的59例婴儿胃肠炎中，有5例观察到WI 61病毒的特征性RNA基因组电泳型，但后来在儿童医院没有观察到。在费城地区收集的大多数正常成人和12月龄婴儿的血清中观察到血清型WI 61特异性中和抗体< 4 or >。对WI61的中值抗体滴度等于或超过对血清型1或3的轮状病毒的中值抗体滴度。7份市售牛奶样品均显示出对WI 61病毒的中和抗体，其滴度大于或等于对血清1型或3型或牛（NCDV株）轮状病毒的中和抗体。然而，WI 61轮状病毒没有诱导疾病或特定的血清中和抗体反应时，喂养剖腹产来源的初乳剥夺新生小牛。WI61以107.0 PFU的50%致痢剂量引起新生小鼠腹泻。
A virus (strain WI61) representing a presumptive new human serotype was isolated from an 18-month-old child with gastroenteritis admitted to Children''s Hospital of Philadelphia [Pennsylvania, USA] in February 1983. The WI61 virus was clearly distinguished by cross-neutralization tests from human rotaviruses of serotypes 1, 2, 3, and 4, human 69M, and representative bovine (NCDV), porcine (OSU), and chicken (Ch2) rotaviruses. Antisera generated in guinea pigs hyperimmunized to WI61 virus displayed a partial cross-reactivity with rotaviruses of human serotypes 1, 2, 3,and 4. By means of studies with reassortant rotaviruses, it was presumptively determined that the WI61 virus cross-reactive antigenic determinants are localized on the vp3 surface polypeptide coded by gene segment 4. The characteristic RNA genome electropherotype of WI61 virus was observed in 5 of 59 cases of infant gastroenteritis detected in 1983 and 1984 but has not been observed subsequently at Children''s Hospital. Serotype WI61-specific neutralizing antibodies were observed in a majority of sera of normal adults and infants of < 4 or > 12 months of age collected in the Philadelphia area. Median antibody titers to WI61 equaled or exceeded those to rotaviruses of serotype 1 or 3. Each of seven samples of commercial cow''s milk exhibited neutralizing antibodies to WI61 virus at a titer greater than or equal to that to serotype 1 or 3 or bovine (strain NCDV) rotavirus. However, WI61 rotavirus did not induce disease or a specific serum-neutralizing antibody response when fed to a caesarean-derived colostrum-deprived newborn calf. WI61 caused diarrhea in newborn mice with a 50% diarrhea-inducing dose of 107.0 PFU.