Enzymatic alteration of the ability of mouse egg plasma membrane to interact with sperm.
Enzymatic alteration of the ability of mouse egg plasma membrane to interact with sperm.
复制标题
酶促改变小鼠卵质膜与精子相互作用的能力。
DOI：
10.1095/biolreprod39.1.19
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Preble,J
中科院分区：
文献类型：
--
作者：
Boldt,J;Howe,AM;Preble,J
The effect of a variety of proteolytic, glycosidic and lipid hydrolyzing enzymes on the ability of mouse egg plasma membrane to interact with sperm was evaluated in this study. Zona-free mouse eggs were exposed to enzymes at various concentrations, washed, and inseminated; the number of sperm attached to or having penetrated the egg plasma membrane was determined at 20 and 180 min post-insemination, respectively. The proteases trypsin and chymotrypsin caused concentration-dependant reductions in both sperm attachment and sperm penetration levels when eggs were incubated at enzyme concentrations ranging from 1 - to 1000 μg/ml for 30 min prior to insemination. Time-course studies revealed significant inhibition of both sperm attachment and sperm penetration levels after treating zona-free eggs for 5 min at 1000 μg/ml of either trypsin or chymotrypsin. Several of the phospholipases tested, including phospholipases C, D, and A2, had no inhibitory effecton sperm penetration levels, with phospholipase C and A2 (100 μg/ml) causing inhibition of sperm attachment. Of the glycosidic enzymes evaluated, glucuronidase (1000 μg/ml) caused significant inhibition of sperm binding but not sperm penetration, and glucosidase, galactosidase, and neuraminidase had no effecton either sperm attachmentor sperm penetration. These findings indicate that the ability of the mouse egg plasma membrane tofuse with sperm can be preferentially altered by treatment with proteases.
