Optical imaging of the intrinsic signal as a measure of cortical plasticity in the mouse
Optical imaging of the intrinsic signal as a measure of cortical plasticity in the mouse
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DOI：
10.1017/s0952523805225178
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发表时间：
2005-09-01
影响因子：
1.9
通讯作者：
Stryker, MP
中科院分区：
文献类型：
--
作者：
Cang, JH;Kalatsky, VA;Stryker, MP
在生命早期的关键时期，单眼视觉剥夺(MD)后，视皮层细胞对双眼刺激的反应发生了巨大的变化。这种现象被称为眼优势(OD)可塑性，是理解皮质可塑性的一个广泛的模型。在这项研究中，我们设计了刺激模式和量化方法，利用内在信号的光学成像来分析小鼠视皮层的光密度。使用限制在双眼视野部分的周期性漂移条，我们获得了对侧(C)和同侧(I)的皮质图，并计算出OD图为(C-I)/(C+I)。我们将单个动物的OD指数(ODI)定义为OD图的平均值。在不到30分钟的成像过程中获得的ODI为正常和单眼剥夺的小鼠在Nembutal麻醉下提供了可靠的OD测量方法。令人惊讶的是，产生出色地形图的乌拉坦麻醉并没有产生一致的OD结果。正常的Nembutal麻醉小鼠有阳性的ODI(0.22+/-0.01)，证实了双眼区的对侧偏向。在关键期内单眼剥夺的小鼠，剥夺眼对侧大脑皮质的ODI向非剥夺的同侧眼负移(2天后ODI：0.12+/-0.02，4天后：0.03+/-0.03，6~7天后ODI：-0.01+/-0.04)。4天MD引起的ODI漂移似乎接近最大，与先前使用单一单位记录的发现一致。因此，我们建立了本征信号的光学成像作为一种快速而可靠的筛选方法来研究小鼠的外径可塑性。
The responses of cells in the visual cortex to stimulation of the two eyes changes dramatically following a period of monocular visual deprivation (MD) during a critical period in early life. This phenomenon, referred to as ocular dominance (OD) plasticity, is a widespread model for understanding cortical plasticity. In this study, we designed stimulus patterns and quantification methods to analyze OD in the mouse visual cortex using optical imaging of intrinsic signals. Using periodically drifting bars restricted to the binocular portion of the visual field, we obtained cortical maps for both contralateral (C) and ipsilateral (I) eyes and computed OD maps as (C - I)/(C + I). We defined the OD index (ODI) for individual animals as the mean of the OD map. The ODI obtained from an imaging session of less than 30 min gives reliable measures of OD for both normal and monocularly deprived mice under Nembutal anesthesia. Surprisingly, urethane anesthesia, which yields excellent topographic maps, did not produce consistent OD findings. Normal Nembutal-anesthetized mice have positive ODI (0.22 +/- 0.01), confirming a contralateral bias in the binocular zone. For mice monocularly deprived during the critical period, the ODI of the cortex contralateral to the deprived eye shifted negatively towards the nondeprived, ipsilateral eye (ODI after 2-day MD: 0.12 +/- 0.02, 4-day: 0.03 +/- 0.03, and 6- to 7-day MD: -0.01 +/- 0.04). The ODI shift induced by 4-day MD appeared to be near maximal, consistent with previous findings using single-unit recordings. We have thus established optical imaging of intrinsic signals as a fast and reliable screening method to study OD plasticity in the mouse.