Cytokeratin expression in non-neoplastic oesophageal epithelium and squamous cell carcinoma of the oesophagus
Cytokeratin expression in non-neoplastic oesophageal epithelium and squamous cell carcinoma of the oesophagus
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DOI：
10.1007/bf00191342
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发表时间：
2004
期刊：
影响因子：
3.5
通讯作者：
K. Lam;Loke Sl;Shen Xc;Lily T Ma
中科院分区：
文献类型：
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作者：
K. Lam;Loke Sl;Shen Xc;Lily T Ma
采用冷冻切片单克隆抗体检测35例食管鳞状细胞癌（高分化10例，中分化13例，低分化12例）及癌旁黏膜细胞角蛋白19、8、18、13、10、7的表达。这项研究是为了评估这些角蛋白在食管肿瘤中的表达模式及其与分化程度的关系。正常食管上皮细胞表达CK19的占86%，表达CK18的占17%，表达CK13的占14%。未观察到CK8、CK10和CK7的免疫反应性。肿瘤表达CK19的占86%，表达CK8的占46%，表达CK18的占97%，表达CK13的占83%，表达CK10的占34%，表达CK7的占29%。因此，所谓的简单上皮标记物CK18和CK19出现在大多数食管鳞状细胞癌中。CK13（所谓的非角化性鳞状上皮标志物）仅在非肿瘤性食管黏膜中少见，其在癌中的表达更为频繁。CK10在非肿瘤性粘膜中未被证实，但主要与高分化癌相关。因此，我们得出结论，食管癌细胞角蛋白的表达模式与正常食管上皮不同，并随分化而变化。
The expression of cytokeratins (CK) 19, 8, 18, 13, 10 and 7 was examined in 35 cases of squamous cell carcinomas of the oesophagus (10 well-differentiated, 13 moderately-differentiated, and 12 poorly-differentiated) and the adjacent mucosa by means of a panel of monoclonal antibodies on frozen sections. The study was undertaken to assess the pattern of expression of these keratins in oesophageal tumours and its relation to the degree of differentiation. The normal oesophageal epithelia expressed CK19 in 86%, CK18 in 17% and CK13 in 14% of cases. CK8, CK10 and CK7 immunoreactivity was not observed. The tumours expressed CK19 in 86%, CK8 in 46%, CK18 in 97%, CK13 in 83%, CK10 in 34% and CK7 in 29% of cases. Thus, the so-called simple epithelial markers CK18 and CK19 occurred in the majority of oesophageal squamous cell carcinomas. CK13 (the so-called non-keratinizing squamous epithelial marker) was only infrequently demonstrated in the non-neoplastic oesophageal mucosa, and its expression was more frequent in carcinomas. CK10 was not demonstrated in non-neoplastic mucosa, but was mostly associated with well-differentiated carcinomas. We therefore conclude that the pattern of expression of cytokeratins in oesophageal carcinomas is different from that in normal oesophageal epithelia and varies with differentiation.