Impact of isoflurane on malignant capability of ovarian cancer in vitro
Impact of isoflurane on malignant capability of ovarian cancer in vitro
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DOI：
10.1093/bja/aeu408
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发表时间：
2015-05-01
影响因子：
9.8
通讯作者：
Ma, D.
中科院分区：
文献类型：
--
作者：
Luo, X.;Zhao, H.;Ma, D.
背景。卵巢癌的转移性复发是术后死亡的首要原因。最近的研究表明吸入麻醉剂可能会影响癌细胞的行为，本研究探讨了异氟烷对卵巢癌细胞致瘤标志物表达和增殖能力的影响。方法。培养卵巢癌 (SK-OV3) 细胞，然后暴露于 2% 异氟烷 2 小时。使用免疫荧光染色、蛋白质印迹和流式细胞术评估治疗后 24 小时内涉及细胞增殖、血管生成和迁移的标志物的表达。还确定了异氟烷对体外血管生成和迁移的影响。结果。异氟烷暴露显着增加 SK-OV3 细胞中胰岛素样生长因子 (IGF)-1 和 IGF-1R 的表达、细胞周期进展和细胞增殖。血管生成标记物血管内皮生长因子 (VEGF) 的表达增加 56% (P
Background. Metastatic recurrence of ovarian cancer is the foremost cause of postoperative mortality. With recent research indicating that inhalation of anaesthetics may influence cancer cell behaviour, this study investigated the effects of isoflurane on the expression of tumorigenic markers and proliferative capacity in ovarian cancer cells.Methods. Ovarian cancer (SK-OV3) cells were cultured and then exposed to 2% isoflurane for 2 h. The expression of markers involved in cell proliferation, angiogenesis, and migration were assessed up to 24 h after treatment using immunofluorescence staining, western blotting, and flow cytometry. The effects of isoflurane on in vitro angiogenesis and migration were also determined.Results. Isoflurane exposure significantly increased insulin-like growth factor (IGF)-1 and IGF-1R expression, cell cycle progression, and cell proliferation in SK-OV3 cells. Increased expression of the angiogenic markers vascular endothelial growth factor (VEGF) by 56% (P