Mediators of hypersensitivity pneumonitis.
Mediators of hypersensitivity pneumonitis.
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DOI：
10.1067/mlc.2000.107694
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发表时间：
2000-07
期刊：
影响因子：
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通讯作者：
Mark Schuyler;K. Gott;A. Cherne
中科院分区：
文献类型：
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作者：
Mark Schuyler;K. Gott;A. Cherne
吸入直脉糖多孢菌（S. rectivirgula）引起农民肺病，这是过敏性肺炎（HP）的典型例子。HP的特征是支气管肺泡灌洗液（BALF）嗜中性粒细胞（在吸入后的前48小时内），随后是BALF淋巴细胞增多。我们利用一个描述良好的小鼠HP模型来确定C-C趋化因子单核细胞趋化蛋白-1（MCP-1）和巨噬细胞炎性蛋白-1 α（macrophage inflammatory protein-1 alpha）出现的时间。（MIP-1 α）;炎症细胞因子肿瘤坏死因子（TNF）、白细胞介素-1 α（IL-1 α）和白细胞介素-6（IL-6）;以及Th 1分化细胞因子白细胞介素-12（IL-12）。单次腹腔注射S.在C57 Bl/6和BALB/c小鼠中，BALF嗜中性粒细胞增多（24 - 48小时达到峰值），随后是BALF淋巴细胞增多（48 - 72小时达到峰值），在此之前，BALF中出现MIP-1 α（4 - 6小时达到峰值）和MCP-1（48小时达到峰值）。在两种品系的小鼠中，BALF IL-12显著增加（峰值48至72小时）。BALF中IL-6、IL-1 α和TNF的增加在BALB/c小鼠中也大于C57 Bl/6小鼠。S. rectivirgula诱导小鼠巨噬细胞系J774A.1分泌MIP-1 α、MCP-1、IL-6、IL-1 α和IL-12;诱导C57 B1/6肺泡巨噬细胞分泌MIP-1 α、IL-6、IL-1 α、IL-12和TNF;诱导BALB/c肺泡巨噬细胞分泌IL-1 α、IL-6和TNF，但不诱导IL-12。结论：腹腔注射S. rectivirgula之前BALF嗜中性粒细胞和淋巴细胞增多症，并可能导致分化的Th 1细胞，我们还得出结论，肺巨噬细胞代表这些物质的潜在来源。
Inhalation of Saccharopolyspora rectivirgula (S. rectivirgula) causes farmer's lung disease, a classic example of hypersensitivity pneumonitis (HP). HP is characterized by bronchoalveolar lavage fluid (BALF) neutrophilia (within the first 48 hours after inhalation), followed by BALF lymphocytosis. We utilized a well-described murine model of HP to determine the timing of the appearance of the C-C chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1alpha (MIP-1alpha); the inflammatory cytokines tumor necrosis factor (TNF), interleukin-1alpha (IL-1alpha), and interleukin-6 (IL-6); and the Th1 -differentiating cytokine interleukin-12 (IL-12) in BALF. After a single intratracheal administration of S. rectivirgula, there was remarkable BALF neutrophilia (peak 24 to 48 hours), followed by a BALF lymphocytosis (peak 48 to 72 hours) in both C57Bl/6 and BALB/c mice that was preceded by the appearance of MIP-1alpha in BALF (peak 4 to 6 hours) and MCP-1 (peak at 48 hours). In both strains of mice there was a striking increase of BALF IL-12 (peak 48 to 72 hours). There was also an increase in BALF IL-6, IL-1alpha, and TNF that was greater in the BALB/c mice than in the C57Bl/6 mice. S. rectivirgula induced the secretion of MIP-1alpha, MCP-1, IL-6, IL-1alpha, and IL-12 from the murine macrophage cell line J774A.1; MIP-1alpha, IL-6, IL-1alpha, IL-12, and TNF from C57Bl/6 alveolar macrophages; and IL-1alpha, IL-6, and TNF-but not IL-12-from BALB/c alveolar macrophages. We conclude that chemokines and cytokines induced by intratracheal administration of S. rectivirgula precede BALF neutrophilia and lymphocytosis and may cause differentiation of Th1 cells; we also conclude that pulmonary macrophages represent a potential source of these substances.