Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp strain US80
Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp strain US80
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DOI：
10.1016/j.resmic.2004.10.006
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发表时间：
2005-04-01
影响因子：
2.6
通讯作者：
Laatsch, H
中科院分区：
文献类型：
--
作者：
Fourati-Ben Fguira, L;Fotso, S;Laatsch, H
从突尼斯绿洲土壤中分离到一株对革兰氏阳性菌和革兰氏阴性菌具有抗菌活性的放线菌US 80。培养特性研究表明该菌株属于链霉菌属。链霉菌US 80菌株16 S rRNA基因的核苷酸序列（1517 pb）与其它链霉菌16 S rRNA基因的核苷酸序列相似性为97-98%。与产生氨基糖苷类抗生素黄霉素的玫瑰色链霉菌的16 S rRNA基因获得了98%的相似性。不同营养化合物对生物活性分子产生的影响的研究表明，当在镁的存在下使用1%（w/v）的葡萄糖作为唯一碳源时，获得最高的抗微生物活性。提取链霉菌菌株US 80的发酵液和各种分离和纯化步骤导致分离三种纯活性分子。根据IR、ESI-MS、H-1和C-13/APT NMR数据并与文献中的参考数据进行比较，确定了这三个化合物的化学结构，命名为irumamycin（1a）、X-14952 B（1b）和17-hydroxy-venturiicidin A（1c）。(c)2004年，Elsevier SAS。All rights reserved.
A new actinomycete strain, designated US80 and producing antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi, was isolated from Tunisian oasis soil. Cultural characteristic studies strongly suggested that this strain belongs to the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1517 pb) of Streptomyces sp. strain US80 exhibited close similarity (97-98%) with other Streptomyces 16S rRNA genes. Similarity of 98% was obtained with the 16S rRNA gene of Streptomyces roseoflavus, which produces the aminoglycoside antibiotic flavomycin. Study of the influence of different nutritional compounds on production of bioactive molecules showed that the highest antimicrobial activities were obtained when glucose at 1% (w/v) was used as sole carbon source in the presence of magnesium. Extraction of fermentation broth of Streptomyces sp. strain US80 and various separation and purification steps led to isolation of three pure active molecules. The chemical structure of these three compounds, named irumamycin (1a), X-14952B (1b) and 17-hydroxy-venturicidin A (1c), was established on the basis on their IR, ESI-MS, H-1 and C-13/APT NMR data and by comparison with reference data from the literature. (c) 2004 Elsevier SAS. All rights reserved.