Critical evaluation of five emissive europium(III) complexes as optical probes: correlation of cytotoxicity, anion and protein affinity with complex structure, stability and intracellular localisation profile
Critical evaluation of five emissive europium(III) complexes as optical probes: correlation of cytotoxicity, anion and protein affinity with complex structure, stability and intracellular localisation profile
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DOI：
10.1039/b803895c
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发表时间：
2008-01-01
影响因子：
3.2
通讯作者：
Parker, David
中科院分区：
文献类型：
--
作者：
Murray, Benjamin S.;New, Elizabeth J.;Parker, David
五种结构相关的含氮杂蒽酮或氮杂蒽酮发色团的七齿酸大环配体铕（III）配合物作为细胞内环境的响应探针进行了比较评价。蛋白质结合（HSA）和氧阴离子结合常数通过滴定分析测量，考察了三个例子的比率发射变化，并确定了对柠檬酸盐或碳酸氢盐具有选择性的系统。每个配体和复合物的细胞毒性已被评估，并与观察到的化学稳定性相关。在竞争性水介质中。L-2的铕配合物是无毒的，其发射光谱pro变化很大。允许比率分析的HCO3-（或柠檬酸盐）浓度的变化，并定位于细胞线粒体。这些特征预示着它未来将作为一种灵敏的探针在显微镜下监测pHCO的局部变化(3)。
Five structurally related europium(III) complexes of heptadentate macrocyclic ligands bearing azaxanthone or azathiaxanthone chromophores have been evaluated comparatively as responsive probes of the intracellular environment. Protein binding (HSA) and oxy-anion binding constants have been measured by titrimetric analysis, examining ratiometric emission changes for three examples, and systems exhibiting selectivity for citrate or hydrogencarbonate identified. The cytotoxicity of each ligand and complex has been assessed and correlated with the observed chemical stability pro. le in competitive aqueous media. The europium complex of L-2 is non-toxic, exhibits a large change in its emission spectral pro. le to variation in HCO3- (or citrate) concentration allowing ratiometric analysis, and localises in cellular mitochondria. Such features augur well for its future application as a responsive probe in microscopy to monitor local changes in pHCO(3).