Differential protein expression in seminal plasma from fertile and infertile males.
Differential protein expression in seminal plasma from fertile and infertile males.
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DOI：
10.4103/0974-1208.142485
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发表时间：
2014-07
影响因子：
--
通讯作者：
Cardona Maya W
中科院分区：
文献类型：
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作者：
Cadavid J AP;Alvarez A;Markert UR;Cardona Maya W
本研究的目的是使用表面增强激光解吸电离飞行时间质谱技术（SELDI-TOF-MS）分析人类精浆蛋白质与男性生育状态的关系。使用常规方法进行精液分析。精浆的蛋白质谱通过强阴离子交换剂ProteinChip® Q10阵列上的SELDI-TOF质谱法获得。我们发现有生育能力和不育男性之间的活动力和精子计数存在统计学显著差异。此外，我们还观察到10种精液蛋白在不育组中表达显著上调。总之，精浆蛋白质组在生育和不育男性的比较提供了新的方面，在男性生育的生理，并可能有助于确定新的男性不育的标志物。
The aim of this study was to analyze human seminal plasma proteins in association with male fertility status using the proteomic mass spectrometry technology Surface-Enhanced Laser Desorption Ionization Time-of-Flight (SELDI-TOF-MS). Semen analysis was performed using conventional methods. Protein profiles of the seminal plasma were obtained by SELDI-TOF mass spectrometry over a strong anion exchanger, ProteinChip® Q10 array. We found statistically significant differences in motility and sperm count between fertile and infertile men. In addition, we observed ten seminal proteins that are significantly up-regulated in the infertile group. In conclusion, comparison of seminal plasma proteome in fertile and infertile men provides new aspects in the physiology of male fertility and might help in identifying novel markers of male infertility.