An evolving hierarchical family classification for glycosyltransferases
An evolving hierarchical family classification for glycosyltransferases
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DOI：
10.1016/s0022-2836(03)00307-3
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发表时间：
2003-04-25
影响因子：
5.6
通讯作者：
Henrissat, B
中科院分区：
文献类型：
--
作者：
Coutinho, PM;Deleury, E;Henrissat, B
糖基转移酶是一组普遍存在的酶，其催化糖部分从活化的糖供体转移到糖或非糖受体上。虽然许多糖基转移酶催化化学上类似的反应，推测通过过渡态与大量的氧碳正离子字符，他们显示显着的多样性，在其供体，受体和产品的特异性，从而产生潜在的无限数量的糖缀合物，寡糖和多糖。我们已经进行了全面的调查，糖基转移酶相关的序列（超过7200至今），并在这里提出了一个分类，这些酶类似于以前提出的糖苷水解酶，到一个层次系统的家庭，氏族，折叠。这种不断发展的分类合理化的结构和机制的调查，利用各种相关酶的信息，告知细胞生物学和克服糖基转移酶相关的开放阅读框的功能预测中经常出现的问题。(C)2003爱思唯尔科技有限公司版权所有。
Glycosyltransferases are a ubiquitous group of enzymes that catalyse the transfer of a sugar moiety from an activated sugar donor onto saccharide or non-saccharide acceptors. Although many glycosyltransferases catalyse chemically similar reactions, presumably through transition states with substantial oxocarbenium ion character, they display remarkable diversity in their donor, acceptor and product specificity and thereby generate a potentially infinite number of glycoconjugates, oligo- and polysaccharides. We have performed a comprehensive survey of glycosyltransferase-related sequences (over 7200 to date) and present here a classification of these enzymes akin to that proposed previously for glycoside hydrolases, into a hierarchical system of families, clans, and folds. This evolving classification rationalises structural and mechanistic investigation, harnesses information from a wide variety of related enzymes to inform cell biology and overcomes recurrent problems in the functional prediction of glycosyltransferase-related open-reading frames. (C) 2003 Elsevier Science Ltd. All rights reserved.