Antibacterial efficacy of Nisin, Pediocin 34 and Enterocin FH99 against Listeria monocytogenes and cross resistance of its bacteriocin resistant variants to common food preservatives.
Antibacterial efficacy of Nisin, Pediocin 34 and Enterocin FH99 against Listeria monocytogenes and cross resistance of its bacteriocin resistant variants to common food preservatives.
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DOI：
10.1590/s1517-83822013005000025
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Malik RK
中科院分区：
文献类型：
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作者：
Kaur G;Singh TP;Malik RK
测试了三种细菌素，即Nisin、Pediocin 34和Enterocin FH 99单独和组合对单核细胞增生李斯特菌ATCC 53135的抗真菌效力。当细菌素成对组合时，观察到更大的抗菌效果，表明组合使用多于一种LAB细菌素比单独使用时具有更高的抗菌作用。开发了对Nisin、Pediocin 34和Enterocin FH 99具有抗性的单核细胞增生李斯特菌ATCC 53135的变体。评估了野生型及其相应抗性变体的细菌素交叉抗性，结果表明对细菌素的抗性可延伸至同一类别内的其他细菌素。对Pediocin 34的耐药性赋予Enterocin FH 99交叉耐药性，但不赋予Nisin交叉耐药性。类似地，对Enterocin FH99的抗性赋予对Pediocin 34的交叉抗性，但不赋予对Nisin的交叉抗性。此外，在肉汤中测定单核细胞增生李斯特菌的乳链菌肽、Pediocin 34和Enterocin FH99抗性变体对低pH、盐、亚硝酸钠和山梨酸钾的敏感性，并与亲本野生型菌株进行比较。Nisin、Pediocin 34和Enterocin FH99抗性变体对低pH、氯化钠、山梨酸钾或亚硝酸钠没有内在抗性。在任何情况下，细菌素抗性单核细胞增生李斯特菌变异体检查比亲本菌株更耐抑制剂。
Antilisterial efficiency of three bacteriocins, viz, Nisin, Pediocin 34 and Enterocin FH99 was tested individually and in combination against Listeria mononcytogenes ATCC 53135. A greater antibacterial effect was observed when the bacteriocins were combined in pairs, indicating that the use of more than one LAB bacteriocin in combination have a higher antibacterial action than when used individually. Variants of Listeria monocytogenes ATCC 53135 resistant to Nisin, Pediocin 34 and Enterocin FH99 were developed. Bacteriocin cross-resistance of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class. Resistance to Pediocin 34 conferred cross resistance to Enterocin FH 99 but not to Nisin. Similarly resistance to Enterocin FH99 conferred cross resistance to Pediocin 34 but not to Nisin. Also, the sensitivity of Nisin, Pediocin 34 and Enterocin FH99 resistant variants of Listeria monocytogenes to low pH, salt, sodium nitrite, and potassium sorbate was assayed in broth and compared to the parental wild-type strain. The Nisin, Pediocin 34 and Enterocin FH99 resistant variants did not have intrinsic resistance to low pH, sodium chloride, potassium sorbate, or sodium nitrite. In no case were the bacteriocin resistant Listeria monocytogenes variants examined were more resistant to inhibitors than the parental strains.