A novel renal carcinoma predisposing gene of the Nihon rat maps on chromosome 10
A novel renal carcinoma predisposing gene of the Nihon rat maps on chromosome 10
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DOI：
10.1111/j.1349-7006.2001.tb02133.x
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发表时间：
2001-11-01
期刊：
影响因子：
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通讯作者：
Sakurai, J
中科院分区：
文献类型：
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作者：
Hino, O;Okimoto, K;Sakurai, J
2000 年，在日本的 Sprague-Dawley (SD) 品系大鼠群体中发现了一种新的遗传性肾细胞癌 (RC) 大鼠模型，并于 2000 年命名为“Nihon”大鼠。本研究旨在利用 113 只回交动物绘制 Nihon 大鼠的 RC 易感基因图谱。我们目前的数据清楚地表明，Nihon 基因与白细胞介素 3 (IL3) 基因 (chi (2)=93.6，Lod 得分=25.16)、致死 (2) 巨型幼虫 (LLGL1) 基因座 (chi (2)=109.0，Lod 得分=31.56) 和肌球蛋白重链、胚胎骨骼肌 (MYHSE) 基因 (chi (2)=90.6， Lod得分=23.87)，位于大鼠10号染色体的远端部分。基因顺序为Eker(Tsc2)基因(位于大鼠10号染色体的近端部分；人染色体16p 13.3)-21.3 cM-IL3基因(人5q23-31)-4.4 cM-Nihon基因-0.9 cM-LLGL1基因座(人) 17p11.2)-4.4 cM-MYHSE 基因（人 17p13.1）。我们还检测到 MYHSE 基因座野生型等位基因的丢失，符合 Knudson 的“两次命中”模型。因此，Nihon大鼠应该具有与肾癌发生相关的新型抑癌基因的突变。
A novel rat model of hereditary renal cell carcinoma (RC) was found in a rat colony of the Sprague-Dawley (SD) strain in Japan, and named the "Nihon" rat in 2000. This study was designed to map the RC susceptibility gene in the Nihon rat using 113 backcross animals. Our present data clearly show that the Nihon gene is genetically linked to interleukin-3 (IL3) gene (chi (2)=93.6, Lod score=25.16), lethal (2) giant larvae (LLGL1) locus (chi (2)=109.0, Lod score=31.56) and myosin heavy chain, embryonic skeletal muscle (MYHSE) gene (chi (2)=90.6, Lod score=23.87), which are located on the distal part of rat chromosome 10. The order of the genes is the Eker (Tsc2) gene (located on the proximal part of rat chromosome 10; human chromosome 16p 13.3)-21.3 cM-IL3 gene (human 5q23-31)-4.4 cM-Nihon gene-0.9 cM-LLGL1 locus (human 17p11.2)-4.4 cM-MYHSE gene (human 17p13.1). We also detected loss of the wild-type allele at the MYHSE locus, fitting Knudson's "two hit" model. Thus, the Nihon rat should have a mutation of a novel tumor suppressor gene related to renal carcinogenesis.