State-dependent release of acetylcholine in rat thalamus measured by in vivo microdialysis
State-dependent release of acetylcholine in rat thalamus measured by in vivo microdialysis
复制标题
通过体内微透析测量大鼠丘脑中乙酰胆碱的状态依赖性释放
DOI：
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发表时间：
1994
影响因子：
5.3
通讯作者：
Peter B. Reiner
中科院分区：
文献类型：
--
作者：
Julie A. Williams;Jeff Comisarow;Jamie C. Day;H. Fibiger;Peter B. Reiner
中桥脑胆碱能神经元长期以来被认为在行为状态控制中起关键作用。特别是，它们与脑电图描记过程和快速眼动（REM）睡眠的产生有关。然而，被鉴定的中桥脑胆碱能神经元的行为特征尚未被明确证实。在试图解决这个问题，在体内微透析被用来监测乙酰胆碱（ACh）的释放在大鼠丘脑，一个主要的投射网站的中桥脑胆碱能神经元的行为状态。由于大鼠的REM期持续时间较短，因此开发了一种方法，用于从清醒、慢波睡眠和REM睡眠的每个个体状态中收集和积累足够大的样品，以允许通过（HPLC-ECD）进行离线分析。探针的位置和胆碱能神经支配的微透析探针附近的来源进行了验证，结合ChAT免疫组化逆行追踪。最后，钠和钙依赖性的乙酰胆碱在丘脑测量进行了测试，使用TTX和无钙透析液。结果表明：（1）丘脑细胞外ACh浓度在清醒和REM睡眠期间都很高，而在慢波睡眠期间显著降低;（2）大多数胆碱能投射到透析探针附近起源于中桥脑被盖;（3）丘脑的ACh释放是由于钠和钙依赖性机制。与以前的一些假设的预测相反，这些结果表明，中脑桥脑胆碱能神经元在清醒和REM睡眠期间都是活跃的。
Mesopontine cholinergic neurons have long been thought to play a key role in behavioral state control. In particular, they have been implicated in the process of EEG desynchrony and in the generation of rapid eye movement (REM) sleep. However, the behavioral profile of identified mesopontine cholinergic neurons has not been unequivocally demonstrated. In an attempt to address this issue, in vivo microdialysis was used to monitor acetylcholine (ACh) release across behavioral state in the rat thalamus, a major projection site of mesopontine cholinergic neurons. Because REM periods in rats are of short duration, a method was developed to collect and accumulate sufficiently large samples from each of the individual states of wake, slow-wave sleep, and REM sleep to permit off-line analysis via (HPLC- ECD). Probe placement and the source of cholinergic innervation to the vicinity of the microdialysis probe were verified using retrograde tracing combined with ChAT immunohistochemistry. Finally, the sodium and calcium dependence of ACh measured in the thalamus were tested using TTX and calcium-free dialysates. The results showed that (1) extracellular ACh concentrations in the thalamus are high during both wake and REM sleep and significantly lower during slow-wave sleep, (2) the majority of cholinergic projections to the vicinity of the dialysis probes originate in the mesopontine tegmentum, and (3) ACh release in the thalamus is due to sodium- and calcium-dependent mechanisms. In contrast to predictions of some previous hypotheses, these results demonstrate that mesopontine cholinergic neurons are active during both wake and REM sleep.
体外血清素使大鼠背侧被盖核中的胆碱能低阈值爆发神经元超极化。
DOI：
10.1073/pnas.89.2.743
发表时间：
1992
影响因子：
11.1
作者：
Luebke,JI;Greene,RW;Semba,K;Kamondi,A;McCarley,RW;Reiner,PB
通讯作者：
Reiner,PB
使用卡巴胆碱荧光微球将神经元输入映射到快速眼动睡眠诱导位点。
DOI：
10.1126/science.2475910
发表时间：
1989
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Quattrochi,JJ;Mamelak,AN;Madison,RD;Macklis,JD;Hobson,JA
通讯作者：
Hobson,JA