SAMPLING TECHNIQUE AND RHEOLOGY OF HUMAN TRACHEO-BRONCHIAL MUCUS
SAMPLING TECHNIQUE AND RHEOLOGY OF HUMAN TRACHEO-BRONCHIAL MUCUS
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DOI：
10.1164/ajrccm/137.3.707
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发表时间：
1988-03-01
期刊：
影响因子：
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通讯作者：
AMYOT, R
中科院分区：
文献类型：
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作者：
JEANNERETGROSJEAN, A;KING, M;AMYOT, R
本工作的目的是：（1）建立一种不受唾液或表面麻醉污染的人气管支气管粘液取样技术，（2）测量其粘弹性。通过局部应用4%利多卡因对下咽部进行局部麻醉后，将双套管微生物标本刷引入放置在气管中的柔性支气管镜中。使刷与支气管粘膜直接接触20 - 30 s，使粘液在其上聚集。然后从刷上刮下粘液样品，并立即用石蜡油覆盖。用磁流变仪技术测定了其粘弹性能。不包括麻醉时间，整个过程花费不到1分钟（从而最大限度地减少咳嗽的影响），并在大约90%的试验中产生足够的粘液用于流变学分析，即，2.1 .+-. 2.1 .+-. 1.5（SD）mg.从20名禁食的健康非吸烟受试者中收集粘液标本;其中17人在几天后返回进行第二次收集。在1 rad/s下粘液机械阻抗（弹性和粘度的矢量和）的值为：对照1，141 ± 0.001。41（SE）;对照组2，155 . ±. 58达因/平方厘米。在不同日期，受试者之间（CV，130%）和同一受试者内（CV，55%）的粘液粘弹性差异很大。在7例受试者中，在静脉注射0.6 mg阿托品后15 min采集粘液样本。这些样品中的粘弹性为708 ± 0.5。147 dyn/cm 2，该值与对照1（p < 0.05）和对照2（p < 0.05）值显著不同。阿托品也减少了75%的收集量。最后，在8名受试者中，粘液粘弹性测定摄入5毫克地西泮后60分钟。所得值为204 ±。151 dyn/cm 2，该值与任一对照值无显著差异，表明支气管镜检查产生的应力似乎不会影响粘液粘弹性。
The aims of this work were: (1) to establish a technique for the sampling of human tracheobronchial mucus not contaminated by saliva or topical anesthesia, and (2) to measure its viscoelastic properties. After local anesthesia of the hypopharynx by topical application of 4% xylocaine, a double-sleeve microbiology specimen brush was introduced into a flexible bronchoscope placed in the trachea. The brush was left in direct contact with the bronchial mucosa for 20 to 30 s to allow mucus to collect on it. The mucus sample was then scraped from the brush and immediately covered with paraffin oil. Its viscoelastic properties were determined by the magnetic microrheometer technique. Excluding the time to anesthetize, the whole procedure took less than 1 min (thus minimizing the effect of cough) and resulted in sufficient mucus for rheologic analysis in approximately 90% of trials, i.e., 2.1 .+-. 2.1 .+-. 1.5 (SD) mg. Mucus specimens were collected from 20 fasting healthy nonsmoking subjects; 17 of them returned for a second collection several days later. Values for mucus mechanical impedance (vector sum of elasticity and viscosity) at 1 rad/s were: Control 1, 141 .+-. 41 (SE); Control 2, 155 .+-. 58 dyn/cm2. There was a large variation in mucus viscoelasticity, both between subjects (CV, 130%) and within the same subject (CV, 55%) on different days. In 7 subjects, mucus samples were collected 15 min after intravenous injection of 0.6 mg atropine. Viscoelasticity in these samples was 708 .+-. 147 dyn/cm2, a value significantly different from Control 1 (p < 0.05) and Control 2 (p < 0.05) values. Atropine also reduced the quantity collected by 75%. Finally, in 8 subjects, mucus viscoelasticity was measured 60 min after ingestion of 5 mg diazepam. The value obtained was 204 .+-. 151 dyn/cm2, a value not significantly different from either control value, indicating that the stress generated by the bronchoscopy does not appear to affect the mucus viscoelastic properties.