Transcription of mouse kappa chain genes: implications for allelic exclusion.
Transcription of mouse kappa chain genes: implications for allelic exclusion.
复制标题
小鼠 kappa 链基因的转录：对等位基因排除的影响。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
M. Weigert
中科院分区：
文献类型：
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作者：
R. Perry;D. Kelley;C. Coleclough;J. Seidman;P. Leder;S. Tonegawa;G. Matthyssens;M. Weigert
将来自多种产生κ的浆细胞瘤的核RNA进行大小分级，并用一系列克隆的探针进行分析，这些探针代表编码可变区（V）、连接区（J）和恒定区（C）的序列以及选定的间插序列。所有的浆细胞瘤都产生一种核RNA组分，其含有V κ和C κ序列以及J κ和C κ之间的间插序列，并且具有取决于表达四个J κ片段中的哪一个的独特大小（即，存在于分泌的κ链中）。这些RNA是kappa mRNA的前体，kappa mRNA是从生产性重排的C kappa基因转录的。一半的浆细胞瘤检查生产，除了一个kappa mRNA前体，约8.4个酶，含有C κ和上游侧翼序列，但缺乏表达的V区序列的离散组件。产生该组分的能力总是与肿瘤基因组中未重排（生殖系）J κ-C κ区的持续存在相关。在两个κ基因座重排的肿瘤中，非生产性等位基因要么是转录沉默的，要么在少数情况下，转录并加工成缺乏V区序列的“片段”mRNA。这些结果表明，等位基因排斥可以在几个水平的基因表达的影响。他们还提供了一些洞察V和C基因元件的相对贡献，这种表达。
The nuclear RNA from a large variety of kappa-producing plasmacytomas was size fractionated and analyzed with a series of cloned probes representing sequences encoding variable (V), joining (J), and constant (C) regions and selected intervening sequences. All of the plasmacytomas produce a nuclear RNA component that contains V kappa and C kappa sequences as well as the intervening sequence between J kappa and C kappa, and that has a distinctive size depending on which of the four J kappa segments is expressed (i.e., is present in the secreted kappa chain). These RNAs are the precursors of kappa mRNAs, which are transcribed from productively rearranged C kappa genes. Half of the plasmacytomas examined produce, in addition to a kappa mRNA precursor, a discrete component of about 8.4 kilobases that contains C kappa and upstream flanking sequences but lacks the expressed V region sequence. The ability to produce this component is always associated with the persistence in the tumor genome of an unrearranged (germline) J kappa-C kappa region. In tumors rearranged at both kappa loci the nonproductive allele is either transcriptionally silent or, in a minority of cases, transcribed and processed into a "fragment" mRNA lacking V region sequences. These results reveal that allelic exclusion can be effected at several levels of gene expression. They also provide some insight into the relative contributions of the V and C gene elements to this expression.