Gene chip/PCR-array analysis of tissue response to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer surfaces in a mouse subcutaneous transplantation system
Gene chip/PCR-array analysis of tissue response to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer surfaces in a mouse subcutaneous transplantation system
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DOI：
10.1080/09205063.2014.939917
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发表时间：
2014-01-01
影响因子：
3.6
通讯作者：
Yamaoka, Tetsuji
中科院分区：
文献类型：
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作者：
Kakinoki, Sachiro;Sakai, Yusuke;Yamaoka, Tetsuji
为了评估生物惰性2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）聚合物的体内异物反应，将具有大表面积的MPC聚合物涂覆的多孔基质植入小鼠皮下7天和28天，评估周围组织反应和渗透到多孔结构中的细胞。 MPC 聚合物表面在多孔基质周围诱导低血管生成和薄封装，并轻微抑制细胞浸润到多孔基质中。 7天后，M1型巨噬细胞特异性基因（CCR7）的表达被MPC聚合物表面抑制，从而抑制炎症细胞因子/趋化因子基因的表达。然而，28天后，这些基因在MPC聚合物表面上的表达高于未涂覆表面。这些发现表明，MPC 聚合物表面成功地抑制了组织反应早期阶段的炎症反应，并且似乎随着时间的推移延缓了炎症反应的发生。
To evaluate the in vivo foreign body reaction to bio-inert 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers, MPC polymer-coated porous substrates, with large surface area, were implanted subcutaneously in mice for 7 and 28 days, and the surrounding tissue response and cells infiltrating into the porous structure were evaluated. The MPC polymer surface induced low angiogenesis and thin encapsulation around the porous substrate, and slightly suppressed cell infiltration into the porous substrate. M1-type macrophage specific gene (CCR7) expression was suppressed by the MPC polymer surface after 7 days, resulting in the suppression of inflammatory cytokine/chemokine gene expression. However, the expression of these genes on the MPC polymer surface was higher than on the non-coated surface after 28 days. These findings suggest that MPC polymer surfaces successfully inhibit inflammatory responses during the early stage of tissue response, and seem to retard its occurrence over time.