Metal Ion Imbalance-Related Oxidative Stress Is Involved in the Mechanisms of Liver Injury in a Rat Model of Chronic Aluminum Exposure
Metal Ion Imbalance-Related Oxidative Stress Is Involved in the Mechanisms of Liver Injury in a Rat Model of Chronic Aluminum Exposure
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DOI：
10.1007/s12011-016-0627-1
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发表时间：
2016-01
影响因子：
3.9
通讯作者：
Yang Yang-Yang;Hong Wang;Yuanxin Guo;Wenjuan Lei;Jianfeng Wang;Xinyue Hu;Junqing Yang;Qin He
中科院分区：
文献类型：
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作者：
Yang Yang-Yang;Hong Wang;Yuanxin Guo;Wenjuan Lei;Jianfeng Wang;Xinyue Hu;Junqing Yang;Qin He
该研究的目的是探讨慢性铝超负荷对大鼠肝功能的影响及其诱导肝组织金属离子水平和氧化应激的病理变化。 Wistar 大鼠每天一次灌胃葡萄糖酸铝（200 mg Al3+/Kg），每周 5 天，持续 20 周。 HE染色用于观察大鼠肝组织的病理变化。采用生化法检测ALT、AST、ALP、GGT水平以及肝脏SOD活性和血浆MDA含量。采用等离子体原子发射分光光度计检测肝组织中Al、Mn、Fe、Zn、Cu离子含量。我们的结果显示慢性铝超载大鼠肝细胞出现明显的空泡变性、颗粒变性和点状坏死。 ALT、AST、ALP 和 GGT 水平显着升高。肝脏SOD活性显着降低，MDA含量显着升高。 Al超载大鼠肝脏中Al、Mn、Fe、Cu含量显着升高，Al超载大鼠血清中Mn、Fe、Zn、Cu含量显着降低。然而，Al超载大鼠血清中的Al水平与对照大鼠血清中的Al水平没有显着差异。这些结果表明，慢性铝超负荷对大鼠肝脏造成明显损害，导致大鼠肝脏和血清中Al、Mn、Fe、Zn、Cu含量失衡。金属离子失衡相关的氧化应激可能参与铝超负荷引起慢性肝损伤的机制。
The objective of the study is to investigate the effects of chronic aluminum overload on rat liver function and its induction of pathological changes in metal ion levels and oxidative stress in hepatic tissues. Wistar rats were intragastrically administered aluminum gluconate (200 mg Al3+/Kg) once a day, 5 days a week, for 20 weeks. HE staining was used to visualize pathological changes in rat liver tissue. A biochemical method was adopted to detect ALT, AST, ALP, and GGT levels, as well as liver SOD activity and blood plasma MDA content. A plasma atomic emission spectrophotometer was used to detect Al, Mn, Fe, Zn, and Cu ion contents in liver tissue. Our results showed obvious vacuolar degeneration, granular degeneration, and spotty necrosis in chronic Al-overload rat hepatocytes. The levels of ALT, AST, ALP, and GGT were significantly increased. Liver SOD activity was significantly decreased, and MDA content was significantly increased. In Al-overload rat liver, Al, Mn, Fe, and Cu contents were significantly increased, and in Al-overload rat serum, Mn, Fe, Zn, and Cu contents were significantly decreased. However, the Al level in Al-overload rat serum was not significantly different from that in control rat serum. These results suggest that chronic aluminum overload causes obvious damage to rat liver and causes imbalances in Al, Mn, Fe, Zn, and Cu levels in rat liver and serum. Metal ion imbalance-related oxidative stress may be involved in the mechanism of chronic liver injury caused by aluminum overload.